Enzymes Lab Report
Enzymes are catalytic proteins that speed up chemical reactions by lowering the activation energy of the reaction. our body cells depend highly on enzymes to complete many important functions, such as digestion and metabolism. Enzymes are selective, in which each enzyme can only speed up a specific reaction. There are molecules that enzymes work with called substrates. substrate bind to an area of an enzyme called active site. There is specificity between the enzyme and substrate that react with. When a specific substrate binds to the active site of an enzyme, the active site undergoes slight conformational change to keep the substrate long enough to be converted to a product. So, the activity of enzymes can be determined by measuring the number
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By taking the reciprocal of both side of this equation, we will get a linear equation for the Lineweaver -Burk …show more content…
Also, to understand that the rate of the reaction can be saturated and it depends on the concentration of the substrate (when [S] [E]). Specifically, we studied the kinetics of the enzyme lactase which is an enzyme that catalyzes the hydrolysis of lactose sugar into glucose and galactose as shown in the following reaction: C12H22O11 + H2O C6H12O12 + C6H12O12
Lactose is the disaccharide found in milk, and it requires lactase enzyme for proper digestion. To study lactase kinetics: first, we constructed a standard curve for Ortho- Nitrophenyl (ONP), by plotting the absorbance vs number of moles in the standard samples. Then, we used our ONP standard curve to determine the amount of product formed in moles. Third, using Lineweaver -Burk plot, we measured Km and Vmax for our lactase tablet (Diary Relief 9000 FCC). We expected that as the substrate concentration increases, the reaction rate will increase until the substrate is large enough and the enzyme is saturated (V max). We also expected that the optimal pH of lactase enzyme might be between (2 – 4) because its function is to help digest milk sugar which occurs in the stomach that has a pH of (2 –
By taking the reciprocal of both side of this equation, we will get a linear equation for the Lineweaver -Burk …show more content…
Also, to understand that the rate of the reaction can be saturated and it depends on the concentration of the substrate (when [S] [E]). Specifically, we studied the kinetics of the enzyme lactase which is an enzyme that catalyzes the hydrolysis of lactose sugar into glucose and galactose as shown in the following reaction: C12H22O11 + H2O C6H12O12 + C6H12O12
Lactose is the disaccharide found in milk, and it requires lactase enzyme for proper digestion. To study lactase kinetics: first, we constructed a standard curve for Ortho- Nitrophenyl (ONP), by plotting the absorbance vs number of moles in the standard samples. Then, we used our ONP standard curve to determine the amount of product formed in moles. Third, using Lineweaver -Burk plot, we measured Km and Vmax for our lactase tablet (Diary Relief 9000 FCC). We expected that as the substrate concentration increases, the reaction rate will increase until the substrate is large enough and the enzyme is saturated (V max). We also expected that the optimal pH of lactase enzyme might be between (2 – 4) because its function is to help digest milk sugar which occurs in the stomach that has a pH of (2 –