Enzyme Lab Write Up

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Vaishnavi Kothapalli
Mrs. Manning
Honors Biology
30 November 2012

TITLE:
The Reaction Rate of Catalase in Various Concentrations of Hydrogen Peroxide

QUESTION:
How long does the catalase take to float to the top of a cup filled with different amounts of hydrogen peroxide concentration?

PREDICTION:
A prediction that can be made for this experiment is that the higher the concentration, the faster time it takes the catalase to react with the solution. As the concentration increases from 1% to 100%, the average reaction rate of the catalase will decrease, as the catalase will speed up the reaction.

MATERIALS:
90 mL clear, plastic Solo cups
filter paper
bottle of regular hydrogen peroxide
1%, 25%, 50%, 75%, and 100% substrate concentration
tweezers
timer
permanent marker
pen/pencil
paper for data

PROCEDURE:
The experiment begins with five beakers full of various substrate concentrations. To begin the lab, gather five plastic, Solo cups and label them 1%, 25%, 50%, 75%, and 100% with the permanent marker. Then, fill each cup with 75 mL of regular hydrogen peroxide. After, lightly dip the filter paper into the 1% substrate concentration till it is completely covered. Further, dip the covered paper into the cup labeled 1% (make sure the filter paper is completely submerged and has sunk to the bottom of the cup). After the filter paper is at the bottom of the cup, begin the timer, and time how long it takes the filter paper to rise to the top. After the paper has risen, gently remove it with tweezers and dispose of it properly. Record the time taken by the filter paper to rise to the top by creating a data table on your paper. Do this again for four more trials and record data. Repeat this process with 25%, 50%, 75%, and 100% substrate concentrations. After finishing up with the experiment, make sure to record your data and calculate the averages for each percent of substrate concentration.

MACROSCOPIC QUALITATIVE OBSERVATIONS:...
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