Enzyme Lab Report

Introduction: Enzymes are biological catalysts that permit cells to carry out the many functions that are required in a living system. Every enzyme has a specific substrate and a specific function. Enzymes alter substrates one of three ways: by adding something to the substrate, removing something from the substrate, or by changing its conformation, otherwise known as its shape. The structure of an enzyme and its ability to function exists because it binds to an active site, thus increasing the reactivity of the enzyme and allowing for the reaction to speed up more quickly. However, the regulation of enzyme activity, which is catered towards attention to sensitivity in changes to catalysis as well speed of a catalyzed reaction, is monitored
The purpose of enzyme kinetics is to determine how fast or efficient an enzyme is as well as how specific an enzyme is. This assists in determining whether a certain factor or treatment affects enzyme efficiency which is inclusive of identification of enzymes and prediction of reactions. Kinetic experiments examine the concentration of a product formed or a substrate consumed per unit of time. Plotting the variation of initial velocity with the substrate often results in a linear graph allowing us to determine the maximum velocity at which it can catalyze the reaction. Therefore, it will be presumed that the result of this experiment by isolating the polyphenol oxidase within the potato extract through the oxidation of 5mM dopa solution with highly concentrated enzymatic preparation of polyphenol oxidase with a buffer as the diluent will indicate a rapid increase in the rate of the reaction as well as the amount of activity present in the potato extract. Proposing that Vmax is proportional to enzyme concentration, a linear relationship should be present and enzymatic activity at varying enzyme and substrate concentrations can be