Preview

Ecological Succession Lab Report

Good Essays
Open Document
Open Document
842 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Ecological Succession Lab Report
Ecological Succession

Abstract: To be able to effectively determine and differentiate the various types of bacteria present during a two week fermentation process of both cabbage and cucumber using a variety of selective media. If fermentation is performed, then the CFU/ml of the bacteria should decrease along with the pH.
Introduction: The fermentation of foods has been a long standing tradition and practice among many civilizations, as it was once a means to keep foods fresh and or edible over long periods of time. Today the process of fermentation has become a living/industry for some, and a hobby for others. For the purposes of this lab it was asserted that there will be no use of vinegar (ascetic acid), and instead merely distilled
…show more content…
Day | Day 0 | Day 2 | Day 7 | Day 9 | Day 14 | pH | 6 | 5.9 | 5.4 | 5.3 | 5.4 …show more content…
For example, the cabbage showed no growth over the course of the 14 day period on both the EC and PS plate, which is a desirable sign that the produce could be eaten. The EC plate is selective towards enteric bacteria, and the PS plate is selective to the species Pseudomonas, both of which are not ideal for consumption. Whereas, the data for the cucumber during the 14 day fermentation process showed growth on the PS for days 0-7 then declined. While the EC displayed a pattern of growth, decline, and on the last day displayed growth. It was also interesting to see the differences between the LSD plate growth between the cabbage and the cucumber. The cabbage showed a gradual increase in the CFU/ml, where the cucumber showed a gradual decline in the CFU/ml over the 2 week span. However, the LSD plate for the cucumber on Day 0 was the only plate that showed selection for Streptococcus. Furthermore, the results for both the anaerobic and aerobic TSA plates showed no significant change or rather the values remained relatively consistent. Lastly, there was nothing erroneous for both WN5 plate results and the cucumber and cabbage both were assumed to contain Pediococcus due to the consistent values present on each plate over the course of the experiment. All in all, the cabbage

You May Also Find These Documents Helpful

  • Good Essays

    This experiment is to observe the significance of food safety. In addition, it will illustrate foodborne sicknesses and how food can be compromised by bacteria. This lab will culture washed and unwashed lettuce, as well as fresh unopened milk, and milk that has been opened for 7 days. The prediction for this lab, is that the unwashed lettuce and the opened milk will have the most bacteria growth. All results are meticulous within the expectations of the lab report.…

    • 630 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Purpose: The purpose of the this experiment is to learn to transfer microorganisms to a nutrient medium to promote growth using aseptic techniques. which are a method used to prevent unwanted organisms from contaminating a medium. We will also learn about different types of culture media, including MRS broth and nutrient broth, learn about oxygen and temperature requirements for microbial growth and how to control microbial growth.…

    • 1308 Words
    • 6 Pages
    Better Essays
  • Good Essays

    Micro Lab Project

    • 747 Words
    • 4 Pages

    It is very important to know not only what types of bacteria are in a sample but also how many of them are present. After a 2010 outbreak, founder Jimmy John Liautaud switched the sandwich chain to clover sprouts after Salmonella illnesses were associated with alfalfa sprouts. According to the Centers for Disease Control and the Food and Drug Administration, unwashed or lightly rinsed vegetables can harbor pathogenic bacteria and have been implicated in numerous food borne infections. Food manufacturers are required by the FDA to monitor the number and type of bacteria in their products. Very few of the things we eat or drink are bacteria free. They merely have greatly reduced numbers of “harmless” bacteria. It is often necessary to determine how many live bacteria are actually in a sample, especially when measuring growth rates or determining disinfectant effectiveness. This involves MacConkey agar which is a selective and differential…

    • 747 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    Lab 4

    • 890 Words
    • 4 Pages

    Purpose: The purpose of this experiment is to Learn and employ aseptic technique, to become familiar with basic requirements of microbial growth, to learn the basic forms of culture media, and to become familiar with methods used to control microbial growth.…

    • 890 Words
    • 4 Pages
    Satisfactory Essays
  • Best Essays

    Ecology Lab 1

    • 3568 Words
    • 15 Pages

    Patterns of plant successional change in Gatineau Park, Old Chelsea, were studied quantitatively. The relations between different successional stages of plant communities and abiotic factors were descried. To test these, we went to the fields, and worked on two transect in groups of 4. We counted the trees and measured the diameter at breast height (dbh) for tree with a dbh value greater than 2cm. The dbh measurements (cm), percentage of fern cover and percentage of bare ground were recorded and used to perform statistical analysis. No significant variations in the distribution of abundant tree species using chi2 analysis and percentage of fern cover using t-test analysis were observed between the compared sites. No significant variations found in the interquadrat analysis, and mean dbh of abundant tree species and percentage of bare ground between the sites compared. The results verified the stated hypothesis and were supported by literature sources.…

    • 3568 Words
    • 15 Pages
    Best Essays
  • Good Essays

    Unknown Microorganism

    • 1335 Words
    • 6 Pages

    The streak for isolation was chosen to see the formation of the different colonies and to see if the bacteria would cause growth on the plate. After observing the plate, it could be seen that the unknown had growth and formation of colonies. The Gram stain was chosen next to determine if the unknown was gram-negative or gram-positive. After looking at the gram stain under the microscope with the oil immersion it could be determined that the unknown was gram-negative due to the fact that the bacteria was pink in color and rod shaped. After determining the unknown was gram-negative, other bacteria’s were able to be eliminated from the options. The Triple Sugar Iron Test was chosen next because the unknown could be tested for glucose fermentation, lactose fermentation, sucrose fermentation and sulfur reduction. If the organism is able to ferment glucose and lactose and/or sucrose it will turn the medium yellow throughout (Leboffe & Burton 329). When the Triple Sugar Iron Test was complete, it showed a yellow butt, yellow slant and gas production. This determined that the unknown ferments glucose and one or both of the other sugars. The gas was produced by fermentation. The color change in the agar brought the unknown down to two options of unknowns, Escherichia coli or Hafnia alvei. The SIM medium test was chosen next to see sulfur reduction, indole production from tryptophan…

    • 1335 Words
    • 6 Pages
    Good Essays
  • Better Essays

    Ecology Final Review

    • 17348 Words
    • 70 Pages

    o The weight or mass of an element is equal to the number of protons and number of neutrons…

    • 17348 Words
    • 70 Pages
    Better Essays
  • Satisfactory Essays

    ecology lab 2

    • 691 Words
    • 4 Pages

    A. A species of vole was found to have a type II functional response. At very low food densities what do you expect to limit feeding rate the most? (3pts)…

    • 691 Words
    • 4 Pages
    Satisfactory Essays
  • Good Essays

    Cellular Respiration Lab

    • 452 Words
    • 2 Pages

    The two types of fermentation that are well known are alcoholic fermentation as well as lactic acid fermentation. Fermentation is vital for many organisms, such as yeast and bacteria, because it allows them to obtain energy required to carry on life processes. Alcoholic fermentation is especially important for human beings, as it is used to produce alcoholic beverages, bread, and many other everyday items that are consumed (Alba-Lois, 2010). On the other hand, lactic acid is a waste product of certain bacteria (Lactobacillales), which is utilized to create many dairy products such as yogurt and cheese. In addition, humans can resort to lactic acid fermentation when oxygen is limited, so it is used as an extra source to obtain oxygen. In our experiment we will be using yeast, a single-celled organism that utilizes sugar as a food source, and it produces energy substances through the breakdown of sugar molecules. Specifically, the type of sugar as a source of food, impacts the speed of fermentation in yeast. In this lab, we will calculate the rate of fermentation in yeast with different solutions of sugar, such as sucrose, fructose, and lactose with glucose being the control. It is important to humans that the yeast uses the best sugar source during fermentation, as it creates important everyday items we consume like bread, alcohol, and…

    • 452 Words
    • 2 Pages
    Good Essays
  • Good Essays

    Ecological Succession Lab

    • 736 Words
    • 3 Pages

    Succession, the process of a community changing over time, can be broken down into sub groups such as ecological, primary, pond, and secondary. Ecological succession being the most basic. In the Succession Lab, we observed a community in a ecosystem. As we recorded the data each class, we observed that succession takes place with rapid speed for in each observation, a new specimen is identified.…

    • 736 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Fermentation Mic

    • 1474 Words
    • 6 Pages

    The goal of the ecological succession lab is to demonstrate succession with the fermentation of cabbage and cucumbers. The cabbage will ferment into sauerkraut and the cucumber will ferment to pickles during fermentation process that will changes the species structure and the community of time. During the fermentation process we watched the pH become more acidic, since the desired bacteria would produce lactic acid. The lactic acid would control by inhibiting the spoilage of the cabbage or the cucumber. This was seen by the increase of the acidity in both of the environment, which was seen as the pH went from a neutral 7.0 to a relatively acidic 4.0. The drop in the pH from the acid production from lactic fermentation, and are know as lactic acid bacteria. (Foster, 2009) Typically, these bacteria are Lactobacillus spp., Streptococcus spp. and Pediococcus spp. seen to have the highest bacterial counts of 4.5 *10 6 to 4.3 *107 CFU/mL on the WN5 (microaerophilic) and the LSD plates for the cabbage in our experiment (Foster, 2009). The bacterial counts were the highest for the lactic acid producing bacteria. The initial salt saturation of 5% with the tap water. The lactic acid bacteria can tolerate the salt and it help deter the unwanted bacteria and aid in the production of acid. The following predictions for the five plates are as follows, the TSA plate will gradually increase and then plateau. Also, the WN5 plate would increase over time and plateau; that was the same case for the LSA plate. The PS and the EC plates would both spike initially in growth and then drop off to less than 1.0*101 CFU/mL…

    • 1474 Words
    • 6 Pages
    Good Essays
  • Better Essays

    biology

    • 603 Words
    • 3 Pages

    The focus of this experiment was to identify unknown bacteria provided. The identification of unknown bacteria is beneficial in many aspects of the research of microorganisms and also aids physicians to treat patients correctly. Various tests were performed to provide the fermentation abilities, presence of particular enzymes, and biochemical reactions. Qualitative observations were made on the tests in identification process. The data from experimentation provided sufficient results to suggest that the identity of unknown bacterium was the gram-positive spore forming bacteria, Bacillus Subtilis.…

    • 603 Words
    • 3 Pages
    Better Essays
  • Satisfactory Essays

    3. A “Set” is the new material studied during the initial 7 days in Round 1 and which is reviewed during each succeeding study day within the following Rounds.…

    • 293 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Ecological Succession 2

    • 1095 Words
    • 9 Pages

    Ecological Succession… 1 Ecological Succession is… • The observed process of change in the species structure of an ecological community over time. • The community begins with relatively few pioneering plants and animals and develops through increasing complexity until it becomes stable or self-perpetuating as a climax community. • Succession occurs in all natural environments. Each environment has a particular name that expresses the nature of their ecological succession: Primary and Secondary 2 Ecological Succession 1st year…

    • 1095 Words
    • 9 Pages
    Good Essays
  • Satisfactory Essays

    Final Exam 2013

    • 619 Words
    • 3 Pages

    Answers must be written in ink. Except where they are expressly required, pencils may be used only for drawing, sketching or graphical work.…

    • 619 Words
    • 3 Pages
    Satisfactory Essays