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Dugesia Tigeneration Experiment

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Dugesia Tigeneration Experiment
Success of Dugesia tigrina regeneration in different water temperatures
Abstract: The experiment at hand aimed to determine if the species Duguesia tigrina has an optimal temperature for regeneration. The experiment used three different spring water treatments of different temperatures. The treatments included a cool 12° treatment, a room temperature 25° treatment, and a warm 30° treatment. Each of the D. tigrina were cut in half, and each half was placed into a designated section of a water treatment, and observed over a one week time period. Three replicates of the experiment were done, each over the one week time period. At the end of three weeks, the average number of successfully regenerated D. tigrina in each treatment was observed. It could be observed that in the room temperature 25° treatment, D. tigrina had the most reproduction success.
Introduction: Dugesia tigrina, most commonly known as the Brown Planarian, are simple, small yet fascinating organisms. D. tigrina are “non-parasitic flatworms that have bilateral symmetry” which means their bodies can be divided into two symmetrical halves (Lobo et al 2012).
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tigrina regeneration was conducted by V. R. Rivera and M.J. Perich. A series of four different experiments were conducted on four planarian species, one of which was D. tigrina. One of the experiments involved the regeneration of D. tigrina in four different water temperatures. D. tigrina were placed in 15°, 22°, 25°, and 30° spring water temperatures and the mean of the D. tigrina regenerated in each were recorded. Out of 30 D. tigrina tested in each treatment, an average of 11.33 regenerated in the 15° spring water, 14.33 in the 22° water, 10.67 in the 25° water and 8.33 in the 30° water. (Rivera V.R. and M.J. Perich, 1994). The results gathered in this previously conducted experiment served as a guideline for choosing the final spring water temperatures for this experiment. It was believed that if similar

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