Atomic Absorption Spectroscopy Application
Introduction
Atomic Absorption Spectrometer (AAS) is an analytical equipment commonly used for the detection of heavy metals in particular sample. The first step to analysis in the AAS is the atomization of the desired element by converting it to a gaseous state. The atoms then absorb the energy emitted from the hollow cathode lamp corresponding to the desired element and a detector reads the signal and records the results. The results may be used quantitatively or qualitatively depending on the specific use of the data collected.
Method of Analysis
Determination of Lead Content in Blood
Lead is a highly toxic contaminant found in the environment commonly caused by the inhalation of pollution or the ingestion of contaminated food and water.
Sample Preparation
The blood sample from 25 patients were added with heparin, a natural anticoagulant, and also added with trichloroacetic acid to precipitate the sample. The samples were centrifuged to separate the components needed from the unnecessary. To avoid interferences during analysis, ammonium pyrrolidinedithiocarbamate (APDC) at Ph 3 was added and then extracted with the organic solvent methyl isobutylketone (MIBK). The lead is now extracted as Pb(APCO)2. The organic samples were detected with a detection limit of 0.1µg/ml.
Results
The results shown to have an equation of the line of y=1.006x – 1.953. The samples were recorded to have 12µg/ml to 90µg/ml of lead concentration in the blood samples.
Conclusion
In conclusion, the study shows accurate and dependable data with regards to the amount of lead concentration in the blood samples.
References
[1] http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1008774/pdf/brjindmed00115-0055.pdf
[2]
Atomic Absorption Spectrometer (AAS) is an analytical equipment commonly used for the detection of heavy metals in particular sample. The first step to analysis in the AAS is the atomization of the desired element by converting it to a gaseous state. The atoms then absorb the energy emitted from the hollow cathode lamp corresponding to the desired element and a detector reads the signal and records the results. The results may be used quantitatively or qualitatively depending on the specific use of the data collected.
Method of Analysis
Determination of Lead Content in Blood
Lead is a highly toxic contaminant found in the environment commonly caused by the inhalation of pollution or the ingestion of contaminated food and water.
Sample Preparation
The blood sample from 25 patients were added with heparin, a natural anticoagulant, and also added with trichloroacetic acid to precipitate the sample. The samples were centrifuged to separate the components needed from the unnecessary. To avoid interferences during analysis, ammonium pyrrolidinedithiocarbamate (APDC) at Ph 3 was added and then extracted with the organic solvent methyl isobutylketone (MIBK). The lead is now extracted as Pb(APCO)2. The organic samples were detected with a detection limit of 0.1µg/ml.
Results
The results shown to have an equation of the line of y=1.006x – 1.953. The samples were recorded to have 12µg/ml to 90µg/ml of lead concentration in the blood samples.
Conclusion
In conclusion, the study shows accurate and dependable data with regards to the amount of lead concentration in the blood samples.
References
[1] http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1008774/pdf/brjindmed00115-0055.pdf
[2]
References: [1] http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1008774/pdf/brjindmed00115-0055.pdf [2] http://vedyadhara.ignou.ac.in/wiki/images/3/36/Unit_11_Applications_of_AAS_and_AES.pdf