Anatomic Pathology Essay
Anatomic Pathology / HER-2 DETECTION BY CISH IN BREAST CANCER
Comparison of Fluorescence and Chromogenic In Situ
Hybridization for Detection of HER-2/neu Oncogene in Breast Cancer
Deepali Gupta, MD,1 Lavinia P. Middleton, MD,2 Marion J. Whitaker, CT(ASCP),3 and Jacki
Abrams, MD1,3
Key Words: HER-2/neu; Fluorescence in situ hybridization; Chromogenic in situ hybridization; Breast cancer; CISH; FISH
DOI: 10.1309/P40P2EAD42PUKDMG
Abstract
Determination of HER-2/neu oncogene amplification has become clinically important in the present management of breast cancer and may have important applications in other areas of clinical oncology and scientific research. In situ hybridization is an extremely accurate and sensitive technique for …show more content…
It also permits direct visualization of the gene amplification in the nuclei and provides an objective count of the genes and chromosomes on a cell-by-cell basis. However, it requires a fluorescence microscope and special training for interpretation. The cost of either of the 2 FDA-approved FISH assays
(just reagents) is almost $100, with considerable cost added by the need to purchase a fluorescence microscope and filters. It also may be difficult to visualize the morphologic features of the tumor cells and to separate in situ from invasive carcinoma when evaluating the amplification product with fluorescence. In addition, fluorescence fades quickly and, therefore, is not a permanent record.
Chromogenic in situ hybridization (CISH) seems to provide an attractive alternative to FISH. The tissue preparation and probe hybridization procedures of FISH and
Comparison of Fluorescence and Chromogenic In Situ
Hybridization for Detection of HER-2/neu Oncogene in Breast Cancer
Deepali Gupta, MD,1 Lavinia P. Middleton, MD,2 Marion J. Whitaker, CT(ASCP),3 and Jacki
Abrams, MD1,3
Key Words: HER-2/neu; Fluorescence in situ hybridization; Chromogenic in situ hybridization; Breast cancer; CISH; FISH
DOI: 10.1309/P40P2EAD42PUKDMG
Abstract
Determination of HER-2/neu oncogene amplification has become clinically important in the present management of breast cancer and may have important applications in other areas of clinical oncology and scientific research. In situ hybridization is an extremely accurate and sensitive technique for …show more content…
It also permits direct visualization of the gene amplification in the nuclei and provides an objective count of the genes and chromosomes on a cell-by-cell basis. However, it requires a fluorescence microscope and special training for interpretation. The cost of either of the 2 FDA-approved FISH assays
(just reagents) is almost $100, with considerable cost added by the need to purchase a fluorescence microscope and filters. It also may be difficult to visualize the morphologic features of the tumor cells and to separate in situ from invasive carcinoma when evaluating the amplification product with fluorescence. In addition, fluorescence fades quickly and, therefore, is not a permanent record.
Chromogenic in situ hybridization (CISH) seems to provide an attractive alternative to FISH. The tissue preparation and probe hybridization procedures of FISH and