Analysis Of RAPD-PCR Fingerprinting For Cajanus Cultivar

Results
RAPD and ISSR fingerprint
In order to evaluate the efficiency of RAPD-PCR fingerprinting for Cajanus cajan L. cultivar, the DNA was used as templates for 7 RAPD primers banding modality. Total number of magnified fragments in the Cajanus cajan L. cultivar value of each primer was represented in Table 2. Total of 26 bands were obtained for all primers. The highest band number was produced with primer OP-A5 and OP-B4, while the lowest band number was created with primer OP-A13. The RAPD banding obtained with 7 RAPD primers are presented in Figure 1a. The size of the magnified fragments was ranged between 220 and 1360 bp length (Table 2).
The banding modality of ISSR-PCR fragments using five qualitative primers to fingerprint the Cajanus cajan L. cultivar were documented. The
The mean percentages of DNA damage in tail, tail length (µm) and the ratio of tail moment were observed to be so closer between experimental non-treated animals and 200 mg FFCC /kg b.wt treated group (Figure 4). On the other hand, the mean percentages of DNA damage in tail, tail length (µm) and the ratio of tail moment were increased highly significantly when mice treated with CP (13.02±0.64%, 3.35±0.2µm, and 0.72±0.06) in comparing with non-treated group (3.91±0.57%, 1.45±0.09µm, and 0.12±0.03) respectively. The concurrent administration of FFCC at different doses to mice followed by CP evoked the reduction of DNA damage. This reduction in DNA damage was recorded to increase gradually by increasing the concentration of FFCC from 50 to 200mg/kg b.wt. The maximum reduction of DNA damage was observed after concurrent administration of 200mg FFCC/kg b.wt and CP to increase highly significantly and was reached to 6.32±0.11%, 1.93±0.03µm, and 0.28±0.003 for percentage DNA damage, tail length and tail moment respectively in comparison with those for animals treated with CP alone (Figure