The Identification of Bambusa Sp
The identification of Bamboo using various PCR and Sequencing Techniques
Abstract
Often the incorrect bamboo species is sold to unsuspecting customers at shops. This can have a disastrous effect on their garden. Three separate and unknown Bamboo leaf samples were taken and were required to be distinguished genetically from one another. Using ITS-PCR DNA amplification techniques, the ITS region DNA was amplified and used in PCR-RFLP and RAPD PCR in order to determine the genetic identity of each sample. Sequencing was performed, and results allowed us to distinguish between samples (to a certain extent.)
Introduction
Bamboos are a group of woody perennial green plants (Wikipedia et al. 2006) that are found in many parts of the world. There are 91 genera and about 1,000 species of bamboo (Wikipedia et al. 2006). They are found in diverse climates, from cold mountains to hot tropical regions. Bamboo is a highly desirable plant grown for many reasons in plantations and gardens around the world. Many reasons it is grown are that it is a beautiful ornamental plant with unique properties. Bamboo is also an extremely strong plant that is light; it is used in many building applications for floorboards, and is also often used in furniture making. There are a number of taller growing species that are effective at blocking out the eyes of pepping toms and nosy neighbors.
There are two main forms of bamboo, each form describing the way in which the bamboo itself spreads. These are known as “clumping” (monopodial) and “running” (sympodial) forms. (Wikipedia et al. 2006) Clumping bamboo species tend to spread underground slowly. Running bamboo species are highly variable in their tendency to spread; this is related to both the species and the soil and climate conditions. Some can send out runners several metres a year, while others can stay in the same general area for long periods. If neglected, they can be invasive over time and can cause problems by moving into
Abstract
Often the incorrect bamboo species is sold to unsuspecting customers at shops. This can have a disastrous effect on their garden. Three separate and unknown Bamboo leaf samples were taken and were required to be distinguished genetically from one another. Using ITS-PCR DNA amplification techniques, the ITS region DNA was amplified and used in PCR-RFLP and RAPD PCR in order to determine the genetic identity of each sample. Sequencing was performed, and results allowed us to distinguish between samples (to a certain extent.)
Introduction
Bamboos are a group of woody perennial green plants (Wikipedia et al. 2006) that are found in many parts of the world. There are 91 genera and about 1,000 species of bamboo (Wikipedia et al. 2006). They are found in diverse climates, from cold mountains to hot tropical regions. Bamboo is a highly desirable plant grown for many reasons in plantations and gardens around the world. Many reasons it is grown are that it is a beautiful ornamental plant with unique properties. Bamboo is also an extremely strong plant that is light; it is used in many building applications for floorboards, and is also often used in furniture making. There are a number of taller growing species that are effective at blocking out the eyes of pepping toms and nosy neighbors.
There are two main forms of bamboo, each form describing the way in which the bamboo itself spreads. These are known as “clumping” (monopodial) and “running” (sympodial) forms. (Wikipedia et al. 2006) Clumping bamboo species tend to spread underground slowly. Running bamboo species are highly variable in their tendency to spread; this is related to both the species and the soil and climate conditions. Some can send out runners several metres a year, while others can stay in the same general area for long periods. If neglected, they can be invasive over time and can cause problems by moving into