Absorption Spectroscopy: Beer-Lambert Law

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Absorption Spectroscopy

Author: Cesar M. Lemas

Lab Partners: Jahnnan Jayesh Patel, Myong Ho Lee & Salem Alsaif

Instructor: Jordan Andrew Mudery

Chem 151, Section 7D

Date Work Performed: October 18th 2012

Date Report submitted: October 25th 2012

Absorption spectroscopy is a practical way to find what the light absorption of a substance is, and whit this data combining the Beer’s law equation you can determine the concentration of different things in a solution, in this case using a orange Gatorade looking the concentration of its dye which is yellow dye #6 and what amount of this Gatorade sample is needed to kill someone. The outcomes in this experiment were that the yellow dye #6 concentration is 5.36 x10-6 M and that the LD50 (lethal dose) is 22,515 L / KG.

In this experiment, we are going to use the Absorption Spectroscopies to determine the level of risk posed to the consumer by the amount of dye present in a commercial food product and how much is needed to get a LD50 of this dye. The determination will be accomplished using a Beer’s Law analysis. In this case using an Orange Gatorade with the yellow dye #6 Absorption Spectrometry is the study light absorbed by molecules. In it white light is cause to pass through a sample and then through a device that breaks the light up into a spectrum. When such light is passed through a sample, under right conditions, the electrons of the sample will absorb certain wavelengths of light, and the device will read the others and compare them to the one in the beginning and that’s how the absorption is calculated. With the data obtained from absorption spectroscopy is most often evaluated using the Beer-Lambert Law. This is how absorption measurements are converted into concentration values. The expected outcomes are that we get the concentration of the dye in the sample in this case the orange Gatorade and determine the LD50 of this sample....
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