Absorption Spectra and the Beer-Lambert Law

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Introduction:

Experiments 1 and 2 main aims are as follows:

• To able to use the Beer-Lambert law to explain the relationship linking concentration and absorbance of the coloured solutions methyl orange and bromophenol blue.

• To be able to rightly operate a spectrophotometer to assess the absorbance at various wavelengths

• To be able to identify the main component parts of a spectrophotometer

• To be able to draw a ‘Standard Curve’ from the data collected

• To be able to calculate the ‘percentage absorption coefficient’ for the solutions methyl orange and bromophenol blue.

Method:

The method carried out for experiments 1 and 2 were as detailed in the booklet. There was no variations from the schedule and did not encountered problems while carrying out the experiments.

Results:

Experiment 1:

|Bromophenol Blue | |Wavelength (nm) |Absorbance |Further Readings to determine ‘ λ max’ | |400 |0.003 |Wavelength (nm) |Absorbance | |420 |0.009 |590 |0.979 | |440 |0.013 |600 |0.972 | |460 |0.019 |610 |0.595 | |480 |0.071 | | | |500 |0.105 | | | |520 |0.195 | | | |540 |0.313 | | | |560 |0.458 | | | |580 |0.797 | | | |600 |0.937 | | | |620 |0.281 | | | |640 |0.002 | | | |660 |0.000 | | | |680 |0.000 | | | |700 |0.001 | | |

Experiment 1:

|Methyl Orange | |Wavelength (nm) |Absorbance |Further Readings to determine ‘ λ max’ | |400 |0.406 |Wavelength (nm) |Absorbance | |420 |0.509 |450 |0.676 | |440...
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