The Digestive System
Exercise 8: Chemical and Physical Processes of Digestion: Activity 1: Assessing Starch Digestion by Salivary Amylase Lab Report Pre-lab Quiz Results You scored 100% by answering 6 out of 6 questions correctly. 1. The substrate for amylase is You correctly answered: e. starch and carbohydrate. 2. Which of the following is true of enzymes? You correctly answered: c. Their activity can be affected by temperature and pH. 3. The reagent IKI tests for the presence of You correctly answered: a. starch. 4. Which of the following is not true of controls? You correctly answered: d. A negative result with a positive control is required to validate the test 5. Which of the following is an end product of starch digestion? You correctly answered: e. maltose and glucose 6. Hydrolases are enzymes that break down large molecules into smaller subunits through the addition of _____________. You correctly answered: b. water
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Experiment Results Predict Question: Predict Question: What effect do you think boiling and freezing will have on the activity of the amylase enzyme? Your answer : c. Boiling will decrease amylase activity and freezing will have no effect. Stop & Think Questions: Tube 2 appears to have the same amount of starch digested as tube 3 because You correctly answered: a. freezing had no effect on the enzyme. Experiment Data: Tube No. 1 2 3 4 5 6 7 8 Reagent 1 Amylase Amylase Amylase Amylase Starch Maltose Amylase Amylase Reagent 2 Starch Starch Starch Water Water Water Starch Starch Reagent 3 pH 7.0 pH 7.0 pH 7.0 pH 7.0 pH 7.0 pH 7.0 pH 2.0 pH 9.0 Treatment Boiled Frozen None None None None None None Time 60 60 60 60 60 60 60 60 Temp. 37 37 37 37 37 37 37 37 IKI + + + + Benedict's ++ ++ ++ + +
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Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Tubes 3, 7, and 8 reveal that You correctly answered: d. amylase activity was highest at pH 7. 2. This activity includes a number of negative
010/23/12
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Experiment Results Predict Question: Predict Question: What effect do you think boiling and freezing will have on the activity of the amylase enzyme? Your answer : c. Boiling will decrease amylase activity and freezing will have no effect. Stop & Think Questions: Tube 2 appears to have the same amount of starch digested as tube 3 because You correctly answered: a. freezing had no effect on the enzyme. Experiment Data: Tube No. 1 2 3 4 5 6 7 8 Reagent 1 Amylase Amylase Amylase Amylase Starch Maltose Amylase Amylase Reagent 2 Starch Starch Starch Water Water Water Starch Starch Reagent 3 pH 7.0 pH 7.0 pH 7.0 pH 7.0 pH 7.0 pH 7.0 pH 2.0 pH 9.0 Treatment Boiled Frozen None None None None None None Time 60 60 60 60 60 60 60 60 Temp. 37 37 37 37 37 37 37 37 IKI + + + + Benedict's ++ ++ ++ + +
010/23/12
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Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Tubes 3, 7, and 8 reveal that You correctly answered: d. amylase activity was highest at pH 7. 2. This activity includes a number of negative