From table 8, the concentration of Nitrate, Nitrite and Ammonia in blank solution(ionized/distilled) were 0 mg/l. Blank solution was served as a reference solution that contains the absorbing solution but not the absorbing species. The uses of blank is to negate the background and improve the result accuracy. For the test of Nitrate, the colour of sample solution changes from colourless to Red where the blank solution still remained colourless throughout the test. This showed that no nitrate present in the blank solution. In the test of Nitrite, the reaction of Nitrite with NitriVer 3 reagent in sample solution produced intermediate diazonium salt. The salt undergoes coupling reaction with chromotropic acid to produce pink coloured complex. The concentration of nitrite is 0.07mg/l, therefore the colour observed is light red due to low concentration of nitrite. For the test of Ammonia, ammonia reacted with salicylate to form 5-aminosalicylate and oxidation of 5-aminosalicylate caused the solution to be green. In the blank solution, it was colourless as no ammonia presence in the solution. In the sample water, concentration of ammonia was 0.27mg/l, the colour observed was dark green. Disccssion
Experimental result shows that concentration of Nitrates, Nitrite and Ammonia were 0.573 mg/l, 0.07 mg/l and 0.27 mg/l respectively. Even though the sample water has been polluted, the nitrate concentration and nitrite concentration were still below the limit, which were 10 mg/l and 1 mg/l for nitrates and nitrites respectively. Nitrification occurred in the water in the pH range of 8 to 9 at temperature range of 20 -25 oC, Dissolve oxygen and suitable bacteria. Therefore, the concentration of nitrite will be increased though nitrification. The ammonia, also below the acceptable limit of 0.5 mg/l, however it is not suitable for drinking because drinking solution contain ammonia cause burns in the mouthm throat and stomach. Presence of ammonia in the sample solution means...
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