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    Immunology Lab Report

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    Radial Immunodiffussion (RID) Christian Crespo 18 October 2013 Immunology Lab Report Purpose of the Experiment: The objective of this experiment is to quantitatively observe the foundational reaction in our Immune system; the Antigen-Antibody interactions. The Ouchterlony procedure is what will be used in this lab to detect nature of the antibody interaction. The orientations of the band will provide more information about the interaction of antibody and antigen

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    Immunology Lab Report

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    BRADFORD ASSAY Calculation Formulas: Absorbance of BSA = A595 nm Raw Data = Average of A595 nm of three wells ÷ 3 Example: A1+A2+A3 ÷ 3 In this case‚ 0.365+0.374+0.453 ÷ 3 = .397 Corrected Data =( (A595 nm well) – BackGround) or (Raw Data – Background) Background = negative control = Bradford Reagent + No Protein Background of this standard curve = A595 nm of well ‘A’ = .397 We used well ‘A’ as our negative control. DATA RAW DATA

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    Immunology

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    Introduction to IMMUNOLOGY COURSE • Subject Agenda* Theoretical part (Lecture): 14.01----18.03----22.04.2013 Practical part (Labwork) • Study Materials: Textbook (David Male and Ivan Roitt-2006-DIR; Abul Abbas-2007AA)‚ Clips and Internet search • Classconversation@gmail.com. Pass: btiu12345 • Evaluation - Midterm Exam‚ Final Exam‚ Labwork - Assignment (Home-work‚ Topic-oriented-In-class discussion‚ Readand-Present Practice) Contact me at: R501‚ IU Building; or via email: ntthoai@hcmiu.edu

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    Immunology

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    Title: Immunology Aim: To visit a microbiology department at the university hospital and acquire detailed information of various antigen-antibody tests eg. ASO‚ haemotoglutination‚ ELISA etc. Method: To listen to presentation made and thus formulate a concise report on that which was discussed and demonstrated. (Please see BC34D Laboratory Manual for more information) Concise Report/Summery: The term serology refers to both the study of general antigen-antibody reactions in a laboratory

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    Immunology

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    85326 Lecture 5 Role of transmembrane signaling in immunorecognition (signal transduction) G Ligand (primary 0 messenger) 0.0 shapeType75fBehindDocument1pWrapPolygonVertices8;18;(8674‚879);(15125‚879);(15125‚145);(21500‚145);(21500‚1540);(14509‚1540);(14509‚21497);(3531‚21497);(3531‚19296);(3607‚19296);(3607‚15187);(2071‚15187);(2071‚2713);(0‚2713);(0‚0);(3223‚0);(3223‚806);(8674‚806)posrelh0posrelv0pib Receptor-ligand binding CYTOSOL Cellular responses Signal

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    Unit 10: Blood/Immunology Case Study Lab What were your three diagnoses? Case 1: Bacterial or viral infection Case 2: Normal blood smear Case 3: Normal blood smear Journal Questions for lab In what ways do normal red and white blood cells differ? White blood cells have a nucleus‚ red blood cells do not‚ white blood cells protect while red blood cells transport‚ red blood cells have a longer life span than white blood cells. Which type of white blood cell would you

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    Immunology

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    Innate Targeting of Pathogens Pathogen Examples Facts Pattern recognition alerted by Viruses flu‚ small pox‚ HIV‚ polio‚ Ebola‚ rhinovirus‚ hepatitis‚ measles Can only reproduce inside cells Foreign nucleic acids (double stranded RNA‚ single stranded DNA‚ foreign methylation patterns)‚ reduced antigen presentation by infected cells So viruses are usually recognized by something unusual in their nucleic acids.(Double RNA‚ normally‚ RNA is a single strain) Bacteria

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    immunology

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    Papa Kwame Pireku Problem set 1 2.2) Antimicrobial peptides are usually produced as inactive propeptides that require cleavage by a protease to complete their activation‚ often by generating a cationic peptide that takes on an amphipathic structure capable of disrupting the cell membrane of a microbe. Also in neutrophils the antimicrobial agents are activated by proteolytic cleavage when these granules fuse with phagosome and encounter neutrophil elastase that has been released from primary granules

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    immunology

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    NEUTROPHILS & MACROPHAGES: Neutrophils & Macrophages play the main role in (SEEK AND DESTROY) the invading agents (microbes‚viruses and other injurious agents) NEUTROPHILS are mature cells that can SEEK AND DESTROY agents even in BLOOD ‚ MACROPHAGES begin their life as immature cells in blood called monocytes ‚ when they enter the tissues they swell and become mature and named MACROPHAGES which are extremely capable of SEEK AND DESTROY intratissue injurious agents. White Blood Cells Enter the

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    Blood/Immunology

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    Unit 10: Blood/Immunology Case Study Lab What were your three diagnoses? 1. Case 1: Pernicious Anemia 2. Case 2: Normal Blood Smear 3. Case 3: Malaria Journal Questions for lab 1. In what ways do normal red and white blood cells differ? Many red blood cells circulate in the bloodstream‚ while the white blood cells leave the blood. 2. Which type of white blood cell would you expect to be most common in a normal blood smear? Neutrophils 3. A differential

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    kuby immunology

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    8536d_ch01_001-023 8/1/02 4:25 PM Page 1 mac79 Mac 79:45_BW:Goldsby et al. / Immunology 5e: Overview of the Immune System chapter 1 T        defense system that has evolved to protect animals from invading pathogenic microorganisms and cancer. It is able to generate an enormous variety of cells and molecules capable of specifically recognizing and eliminating an apparently limitless variety of foreign invaders. These cells and molecules act together

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    Medical Immunology

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    MEDICAL IMMUNOLOGY & SEROLOGY Terence L. Eday‚ RMT‚ MT(ASCPi)‚ MPH College of Medical Technology / Medical Laboratory Science University of Perpetual Help System DALTA Historical Perspective • 1773‚ Voltaire reported on an ancient Chinese custom where dried and powdered small pox scabs were inhaled • 1798‚ Edward Anthony Jenner‚ Smallpox vaccination • 1862‚ Ernst Haekel‚ Recognition of phagocytosis • 1877‚ Paul Erlich‚ recognition of mast cells Historical Perspective • 1879‚

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    Lab report

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    Lab #4: The Immune System Purpose: The purpose of this lab was to perform and understand the procedures of conducting an ELISA test to determine whether a particular antibody is present in a patient’s blood sample through a virtual simulation. Hypothesis: If I successfully complete this lab‚ I will then understand how to perform an ELISA test‚ the purpose an ELISA test‚ and also how to interpret the results of this test. Materials and Procedures: Materials: Howard Huges medical

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    Lab Report

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    The Virtual Lab – ELISA Test Lab: Immunology 09/04/2013 Instructors: Dr. Charlie Wilson Written by: Dipen Patel I. Objective: The purpose of the lab was to learn the procedure of performing an ELISA test to determine whether a particular antibody is present in a patient’s blood sample. ELISA is an abbreviation for “Enzyme-linked Immunosorbent Assay." II. Introduction: The interaction of antigen and antibody outside the body can be used to determine if patient

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    Immunology Notes

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    BSCI422 Immunology January 26‚ 2010 Lecture 1 Introduction to the class Went over syllabus Student expectations BSCI422 Immunology January 27‚ 2010 Lecture 2 Innate Immunity‚ the signal transduction and cytokine production When you’re exposed to a microbe‚ your body has innate immune responses that can kill the microbe right away‚ there is no recovery or memory period. It is the very first response. If you amount an affective innate immune response‚ you don’t need to develop an adaptive

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    lab report

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    CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive

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    Lab Reports

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    Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and

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    Lab Report

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    Chem 105 Guide to the Formal Laboratory Report The purpose of a formal report is to communicate effectively to another person the goal‚ procedure‚ data analysis method‚ and results of your laboratory work. The report is divided into several well-defined sections. Each section must be present in a complete report. To earn an outcome point for the laboratory report‚ a student must submit a formal lab report that earns a score of at least 90/100. Each error (factual‚ grammatical‚ typographical

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    lab report

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    Criteria 6 8 10 Title Your lab report has a Title that is directly related to the lab experiment/ exercise. Your group or class must be able to come up with your own title You must make your own title based on scientific theory Aim You have clearly stated your aim Your aim is relevant and informative You must make your own aim based on scientific theory Background background information relevant to the lab experiment/ exercise You must be able to link your aim to the background The

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    FACULTY OF APPLIED SCIENCES BACHELOR OF SCIENCE (HONS.) BIOLOGY BMS 545 (IMMUNOLOGY) TITLE: ETHICAL ISSUES IN IMMUNOLOGY LECTURER’S NAME: PROF ASSOC. DR NOOR HANA BT HUSSAIN GROUP MEMBERS: NURUL ANATI BT CHE OMAR (2010892138) GROUP: AS2014B2 In discussing the ethical issues of using animals in immunological research‚ the major issue is that many of these research animals suffer in ways which are unnatural to them. By using the genetic manipulation‚ obese mice‚ diabetic mice‚ and mice

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