Optimum Temperature Lab Report
RESULTS
28⁰C was the optimal temperature. 28⁰C had the maximum rate of reaction for the class data and results provided by this experiment; the results were represented graphically in graph 1 and graph 2. The reason for having 2 tables and graphs is because 28⁰C was done twice. Data for 28⁰C was collected in this experiment and it was collected again by peers in the classroom. Results are identical because all temperatures except 28⁰C was collected once from peers.
Based on the results from the graphs, at extreme temperatures such as 0⁰C which is extremely cold and 100⁰C which is tremendously hot; catalase activity performed better at 0⁰C than at 100⁰C. Strangely, 50⁰C outperformed 37⁰C. Catalase activity was higher for intermediate temperatures such as 37⁰C and 50⁰C than the extreme temperatures such as 0⁰C and 100⁰C. Overall the rate of reaction peaked at 28⁰C and every other temperature had a lower maximum rate of reaction.
DISCUSSION
The results do not support the hypothesis that optimum temperature would be at 37⁰C. The results do not reflect the textbook narrative of enzyme activity. Enzymes are supposed to peak at optimum temperature and then temperatures further away from the optimum temperature should result in decreased rate of reaction. …show more content…
Nevertheless, enzyme activity peaked at 28⁰C and there was a reduced rate of reaction among all the other temperatures. The graphed results of the average maximum rate of reactions for different temperature shows 28⁰C having the top rate reaction in lab experiment and class data. The result gotten for 37⁰C and 50⁰C are strange because the rate of reaction declines as the temperature is warmer than 28⁰C but it goes back up at 50⁰C.
This means the cooler 37⁰C is a worse condition for catalase than 50⁰C. In normal circumstances, temperatures away for ideal temperature put a strain of the hydrogen bonds that hold the active site on enzymes. This can lead to breaking of the hydrogen bonds that maintain the shape of the active cite. Rate of reaction is supposed to decreased continuously as more enzymes cannot form enzymes-substrate complex because of a denature active sites as a result of higher kinetic energy supplied to the enzymes from raising the temperature beyond the optimal
temperature
Taken as a whole, temperatures closer to 42.8⁰C such as 28⁰C, 37 ⁰C and 50⁰C outperformed extreme temperatures such as 0⁰C and 100⁰C. Enzymes needs kinetic energy to surpass the activation energy to perform biochemical reactions. 0⁰C provides a much smaller amount of kinetic energy than 28⁰C so consequently the rate of reaction is much slower. 100⁰C supplies vast amount kinetic energy beyond catalase optimal temperature which breaks the enzyme’s hydrogen bonds that hold the shape of its active site. The denatured catalase cannot break hydrogen peroxide to release oxygen. This explains why there is smallest catalase activity at 100⁰C.
Human error could be created because humans are not robots so there may be a few seconds’ delay in attempting to hook up the experiment’s mixture to gas sensor and clicking play to start recording the experiment data on the computer. This few seconds error could have happened throughout the classroom. The lack precision could have been reflected in the results.
Catalase is an enzyme that can remains intact in cold extreme temperatures and it resumes metabolic activities when the enzyme has had enough kinetic energy to proceed up to 50⁰C without being denatured. This allows plants and animals to be able to preserve and perform catalase activity as body temperature change to suit the environment.
ACKNOWLEDGMENTS
I thank my lab partner Dido Mangafas for aiding me collect data for this report, my classmates for helping me in collecting data for the other temperatures and my class teacher Dr. Regina Suvelliman for supervising the experiment.
28⁰C was the optimal temperature. 28⁰C had the maximum rate of reaction for the class data and results provided by this experiment; the results were represented graphically in graph 1 and graph 2. The reason for having 2 tables and graphs is because 28⁰C was done twice. Data for 28⁰C was collected in this experiment and it was collected again by peers in the classroom. Results are identical because all temperatures except 28⁰C was collected once from peers.
Based on the results from the graphs, at extreme temperatures such as 0⁰C which is extremely cold and 100⁰C which is tremendously hot; catalase activity performed better at 0⁰C than at 100⁰C. Strangely, 50⁰C outperformed 37⁰C. Catalase activity was higher for intermediate temperatures such as 37⁰C and 50⁰C than the extreme temperatures such as 0⁰C and 100⁰C. Overall the rate of reaction peaked at 28⁰C and every other temperature had a lower maximum rate of reaction.
DISCUSSION
The results do not support the hypothesis that optimum temperature would be at 37⁰C. The results do not reflect the textbook narrative of enzyme activity. Enzymes are supposed to peak at optimum temperature and then temperatures further away from the optimum temperature should result in decreased rate of reaction. …show more content…
Nevertheless, enzyme activity peaked at 28⁰C and there was a reduced rate of reaction among all the other temperatures. The graphed results of the average maximum rate of reactions for different temperature shows 28⁰C having the top rate reaction in lab experiment and class data. The result gotten for 37⁰C and 50⁰C are strange because the rate of reaction declines as the temperature is warmer than 28⁰C but it goes back up at 50⁰C.
This means the cooler 37⁰C is a worse condition for catalase than 50⁰C. In normal circumstances, temperatures away for ideal temperature put a strain of the hydrogen bonds that hold the active site on enzymes. This can lead to breaking of the hydrogen bonds that maintain the shape of the active cite. Rate of reaction is supposed to decreased continuously as more enzymes cannot form enzymes-substrate complex because of a denature active sites as a result of higher kinetic energy supplied to the enzymes from raising the temperature beyond the optimal
temperature
Taken as a whole, temperatures closer to 42.8⁰C such as 28⁰C, 37 ⁰C and 50⁰C outperformed extreme temperatures such as 0⁰C and 100⁰C. Enzymes needs kinetic energy to surpass the activation energy to perform biochemical reactions. 0⁰C provides a much smaller amount of kinetic energy than 28⁰C so consequently the rate of reaction is much slower. 100⁰C supplies vast amount kinetic energy beyond catalase optimal temperature which breaks the enzyme’s hydrogen bonds that hold the shape of its active site. The denatured catalase cannot break hydrogen peroxide to release oxygen. This explains why there is smallest catalase activity at 100⁰C.
Human error could be created because humans are not robots so there may be a few seconds’ delay in attempting to hook up the experiment’s mixture to gas sensor and clicking play to start recording the experiment data on the computer. This few seconds error could have happened throughout the classroom. The lack precision could have been reflected in the results.
Catalase is an enzyme that can remains intact in cold extreme temperatures and it resumes metabolic activities when the enzyme has had enough kinetic energy to proceed up to 50⁰C without being denatured. This allows plants and animals to be able to preserve and perform catalase activity as body temperature change to suit the environment.
ACKNOWLEDGMENTS
I thank my lab partner Dido Mangafas for aiding me collect data for this report, my classmates for helping me in collecting data for the other temperatures and my class teacher Dr. Regina Suvelliman for supervising the experiment.