Optimum Temperature Lab Report
RESULTS
28⁰C was the optimal temperature. 28⁰C had the maximum rate of reaction for the class data and results provided by this experiment; the results were represented graphically in graph 1 and graph 2. The reason for having 2 tables and graphs is because 28⁰C was done twice. Data for 28⁰C was collected in this experiment and it was collected again by peers in the classroom. Results are identical because all temperatures except 28⁰C was collected once from peers.
Based on the results from the graphs, at extreme temperatures such as 0⁰C which is extremely cold and 100⁰C which is tremendously hot; catalase activity performed better at 0⁰C than at 100⁰C. Strangely, 50⁰C outperformed 37⁰C. Catalase activity was higher for intermediate temperatures such as 37⁰C and 50⁰C than the extreme temperatures such as 0⁰C and 100⁰C. Overall the rate of reaction peaked at 28⁰C and every other temperature had a lower maximum rate of reaction.
DISCUSSION
The results do not support the hypothesis that optimum temperature would be at 37⁰C. The results do not reflect the textbook narrative of enzyme activity. Enzymes are supposed to peak at optimum temperature and then temperatures further away from the optimum temperature should result in decreased rate of reaction. …show more content…
This means the cooler 37⁰C is a worse condition for catalase than 50⁰C. In normal circumstances, temperatures away for ideal temperature put a strain of the hydrogen bonds that hold the active site on enzymes. This can lead to breaking of the hydrogen bonds that maintain the shape of the active cite. Rate of reaction is supposed to decreased continuously as more enzymes cannot form enzymes-substrate complex because of a denature active sites as a result of higher kinetic energy supplied to the enzymes from raising the temperature beyond the optimal
28⁰C was the optimal temperature. 28⁰C had the maximum rate of reaction for the class data and results provided by this experiment; the results were represented graphically in graph 1 and graph 2. The reason for having 2 tables and graphs is because 28⁰C was done twice. Data for 28⁰C was collected in this experiment and it was collected again by peers in the classroom. Results are identical because all temperatures except 28⁰C was collected once from peers.
Based on the results from the graphs, at extreme temperatures such as 0⁰C which is extremely cold and 100⁰C which is tremendously hot; catalase activity performed better at 0⁰C than at 100⁰C. Strangely, 50⁰C outperformed 37⁰C. Catalase activity was higher for intermediate temperatures such as 37⁰C and 50⁰C than the extreme temperatures such as 0⁰C and 100⁰C. Overall the rate of reaction peaked at 28⁰C and every other temperature had a lower maximum rate of reaction.
DISCUSSION
The results do not support the hypothesis that optimum temperature would be at 37⁰C. The results do not reflect the textbook narrative of enzyme activity. Enzymes are supposed to peak at optimum temperature and then temperatures further away from the optimum temperature should result in decreased rate of reaction. …show more content…
This means the cooler 37⁰C is a worse condition for catalase than 50⁰C. In normal circumstances, temperatures away for ideal temperature put a strain of the hydrogen bonds that hold the active site on enzymes. This can lead to breaking of the hydrogen bonds that maintain the shape of the active cite. Rate of reaction is supposed to decreased continuously as more enzymes cannot form enzymes-substrate complex because of a denature active sites as a result of higher kinetic energy supplied to the enzymes from raising the temperature beyond the optimal