Effect of temperature on enzyme activity

Topics: Temperature, Celsius, Enzyme, Liver, Fahrenheit, Protein / Pages: 3 (529 words) / Published: Mar 5th, 2013
Lab Report
Effect of temperature on enzyme activity

Determine how effect of temperature on enzyme (catalyse) activity (in the liver).

II. Materials

* Raw liver

* Forceps

* 50mL 1% hydrogen Peroxide (H

* 25mL graduated cylinder

* 50mL, 400mL beakers

* Pureed liver

* 5 Filter-paper disks

* Paper towels

* Timer or stopwatch

* Thermometer

* Hot plate

III. Procedure
1.Gather all necessary materials; start water bath with 120 mLs of water in the 400 mL beaker.
2.Using the graduated cylinder, pour twenty-five mLs of H2O2 into the 50 mL beaker
3.Gently place the beaker of H2O2 into the water bath; heat the H2O2 to thirty-seven degrees Centigrade, or ninety-eight point six degrees Fahrenheit
4.Once the solution is at the desired temperature, with the forceps, dip a filter-paper disk into the pureed liver and place on a paper towel to remove excess liquid
5.Using the forceps, place the filter-paper at the bottom of the H2O2 solution and release it.
6.Using the stopwatch, keep track of the amount of time it takes for the filter-paper to float to the top of the solution.
7.Record the time it takes for the filter-paper to reach the top of the solution in a data table.
8.Repeat steps four through seven three more times, changing the H2O2 solution when necessary.

IV. Data Temperature | Time | Observation | 4C | 0 | Instantly up | 23C | 5.2 | Room temperature | 37C | 0 | Floated on the top instantly | 40C | 0 | Floated right away | 93.3C | 0 | Floated quickly |

V. Result

1. The rate of the reaction was affected by the temperature in such a way that the time it took the reaction to occur significantly decreased. 2. The increase in temperature helped the enzyme react with the liver faster than it did at colder temperatures; The enzyme was most active at the normal temperature of the human body, which is significant

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