The glutamate residue, which is negatively charged, present at 285th position is essential to maintain the aspartoacylase function and also support the catalytic function and is located near the enzyme active site. 15-18 Computational analysis of mutation E285A could bring new insights into reasons underlying pathogenesis of the disease. 19, 20 On the whole, this study would help us understanding the structural and functional defects of the enzyme and disease causing mutation. We have analyzed native and mutant structures to understand the deleterious effects through conformation sampling approach which is an alternate to classical molecular dynamics. The study also gains interest since there are no prior molecular dynamics analyses for the disease. Moreover, this work could direct the implementation of nanomechanics to understand the misfolding nature of protein by the deleterious effect of missense
The glutamate residue, which is negatively charged, present at 285th position is essential to maintain the aspartoacylase function and also support the catalytic function and is located near the enzyme active site. 15-18 Computational analysis of mutation E285A could bring new insights into reasons underlying pathogenesis of the disease. 19, 20 On the whole, this study would help us understanding the structural and functional defects of the enzyme and disease causing mutation. We have analyzed native and mutant structures to understand the deleterious effects through conformation sampling approach which is an alternate to classical molecular dynamics. The study also gains interest since there are no prior molecular dynamics analyses for the disease. Moreover, this work could direct the implementation of nanomechanics to understand the misfolding nature of protein by the deleterious effect of missense