Enzyme Immobilization Methods Covalent Binding: Covalent binding is a conventional method for immobilization; it can be achieved by direct attachment with the enzyme and the material through the covalent linkage [37]. The covalent linkage is strong and stable and the support material of enzymes includes polyacrylamide‚ porous glass‚ agarose and porous silica [38]. Covalent method of immobilization is mainly used when a reaction process does not require enzyme in the product‚ this is the criteria
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College General Biology I Enzymes‚ test for effect of pH on catalase activity Purpose The main purpose of this experiment was to learn about enzymes and how to test for the effect of pH on catalase activity and to be able to tell if a reaction is an exergonic or endergonic process. Introduction Enzymes are made from amino acids‚ which are made from proteins. In order to make an enzyme‚ hundreds of amino acids are strung together in a very specific and unique order and eventually is folded
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TASK 1: The main components of a healthy diet. How the diet and energy requirements of the following individuals vary to meet their specific needs: - An 18 month old baby - A 17 year old girl - A man/woman working physically in the building trade - A pregnant woman - A lactating woman - A 70 year old man/woman. TASK 2: Explain the mechanical and chemical digestion. (a) Ingestion (b) Digestion (c) Absorption (d) Assimilation (e) Egestion TASK 3: The process of
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Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the following substrates: Caffeic Acid‚ Catecol‚ Guaicol‚ Pyragallol and Tyrosine. 3. To determine the effect of ascorbic acid on Peroxidase. 4. To determine the level of specificity of Peroxidase using the following substrates: Caffeic Acid‚ Catecol‚ Guaicol‚ Pyragallol and Tyrosine. 5. To calculate the effect of the substrate acetaldehyde concentration on a Xanthine Dehydrogenase catalyzed reaction. 6. To determine the
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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Meerra Gandi‚ Erin Barody‚ Samantha Gutcho Title: The Effect of Adjusted Concentration of Hydrogen Peroxide on the change in reaction rate of liver catalase. Hypothesis: Null Hypothesis: If the concentration of the Hydrogen Peroxide is changed then there would be no change in the reaction rate. Alternate Hypothesis 1: I there is an increase in concentration in concentration of Hydrogen Peroxide then the reaction rate of the liver catalase will increase. Alternate Hypothesis 2: If there is an increase
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My lab group studied the effect of PH on reaction rate/ enzyme activity measured by foam height. PH is the measure of the concentration of hydrogen ions in a solution. The higher the hydrogen ion concentration‚ the lower the pH. Every enzyme has an optimal PH‚ meaning they have a very small window in which they are most active. Our enzyme (potato smoothie) had an optimal PH of 7.0-7.5. We know this because we measured the enzyme’s reaction rate by measuring foam height. The largest foam height we
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In this lab we used a solution of lactase to test the chemical and physiological properties of this particular enzyme and determined whether the lactase came from human cells or bacterial cells. In the statistical analysis statistical formulas and techniques are used to analyze the significance of a set of data and the validity of the conclusions made based on that data. These are some terms and definitions that will be crucial to understanding the validity of this experiment. An enzyme is a protein
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Enzymes Enzymes are the sparks that start the essential chemical reactions our bodies need to live. They are necessary for digesting food‚ for stimulating the brain‚ for providing cellular energy‚ and for repairing all tissues‚ organs‚ and cells. There are three types of enzymes: metabolic enzymes‚ digestive enzymes‚ and food enzymes. Metabolic enzymes catalyse‚ or spark‚ the reactions within the cells. The body’s organs‚ tissues and cells are run by metabolic enzymes. Without them our bodies
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What effect does temperature have on the oxygen production of enzymes in an animal’s liver? A temperature which is too high would be able to produce little to no oxygen because of the extreme temperature. Just like a temperature too high‚ a temperature which is too low would also produce little to no oxygen for the same reason. Animals that have livers are considered warm-blooded‚ with very warm temperatures; therefore it would make sense for a warm temperature to produce the most oxygen; but one
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