In the first part of this lab‚ E.coli cells were transformed with an R-plasmid carrying a tetracycline resistant gene‚ giving rise to tetracycline resistant E.coli strain. This was accomplished through transformation‚ which allowed E.coli to directly uptake the naked DNA molecule carrying the antibiotic resistant gene (1). However‚ in order to take up the DNA and incorporate them into their genome via recombination‚ cells must be competent (1). Therefore‚ E.coli cells which are not competent under
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SIGNIFICANCE OF ENVIRONMENTAL ANALYSIS By Akshay Saxena Under the Guidance of Mr. Bhende‚ Lecturer in Management‚ Hind Seva Parishad’s Public Night Degree College‚ Mumbai CONTENTS Introduction 3 Meaning & Definition 6 History 7 Importance 8 Types of Business Environments 9 Techniques of Environmental Analysis 11 Swot Analysis 13 Case Study 14 INTRODUCTION Business
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The Apex of Last Days‚ by Luke Orlando Gus Van Sant’s Last Days follows a rock star (introduced only as Blake) through his last two days alive as he struggles to cope with the reality of fame and his resulting alienation from the rest of the world. This particular scene‚ which occurs at the exact midpoint of the film‚ depicts two separate sequences. The first sequence shows Blake escaping his house in order to avoid talking to a friend‚ and the second sequence is a long‚ drawn out shot of Blake
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THE SIGNIFICANCE OF INFORMATION TECHNOLOGY IN THE BUSINESS OF NIGERIA CHEMICAL CORPORATION BY OCHALA YUSUF OJIH MAT. NO: PGSS/ UJ/00442/07 BEING AN ASSIGNMENT ON MBA 505 (ICT MANAGEMENET) SUBMITTED TO MR. OKI‚ E.U.(MBA) DEPARTMENT OF MANAGEMENT SCIENCES UNIVERSITY OF JOS FEBRUARY 2009 INTRODUCTION Since the start of the 21st century‚ there has been an emphasis economic reform in Africa‚ which makes regional economies more attractive to foreign investors: Over the
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fighting for the passage of the Nineteenth Amendment‚ the first wave of the Women’s Movement focused their efforts on more than the right to vote. During the time period within which the Women’s Movement took place‚ women had little to no power in nearly all aspects of life. From having a voice in government to having a voice in their own home‚ women were not regarded with respect and did not have many rights in the eyes of the government. First addressed by Elizabeth Cady Stanton during the Seneca Falls
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America became a country on July 4th‚ 1776; over 240 years ago. That’s greater than 240 years of generations of people trying to define what it means to be an American‚ but few still stand true today from that long ago. Being accepting of others‚ not only other ethnic groups but fellow Americans as well‚ is one way in which things have not changed from the first American generation to the latest. One of the longest standing traits of the “poster child” American is being courageous‚ whether it be
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The Adventures of Huckleberry Finn wherein the title succinctly tells what the story shall contain‚ Kate Chopin’s The Awakening represents a work whose title can only be fully understood after the incorporation of the themes and content into the reader’s mind‚ which can only be incorporated by reading the novel itself. The title‚ The Awakening‚ paints a vague mental picture for the reader at first and does not fully portray what content the novel will possess. After thorough reading of the novel‚
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Gods Must Be Crazy Script - Dialogue Transcript Voila! Finally‚ the Gods Must Be Crazy script is here for all you fans of the 1980 movie. This script is a transcript that was painstakingly transcribed using the screenplay and/or viewings of Gods Must Be Crazy. I know‚ I know‚ I still need to get the cast names in there and I’ll be eternally tweaking it‚ so if you have any corrections‚ feel free to drop me a line. You won’t hurt my feelings. Honest. Swing on back to Drew’s Script-O-Rama afterwards
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these verses it is discussing what is thought to happen in the last judgement when we are all before God one last time. In the Last Judgement it is thought that when it comes about we will all “get what is coming for us” meaning that depending on if we tried to be the best people we could be when we were alive or the worst then we will be judged accordingly. Michelangelo seems to have painted this according to more of the Catholic belief system and what they think the Last Judgement will look like.
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First Ionisation energy is the measure of energy needed to remove a mole of electrons from a mole of gaseous atoms to form a mole of positively charged gaseous ions‚ also known as a cation. The general trend is that as the atomic number increases between elements Lithium to Neon the level of the first ionisation energy increases‚ as there is a stronger nuclear attraction. [Braid‚ K. et al. (2015)]. The stronger nuclear attraction along the period is caused by the number of protons increasing. When
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