Lab Report #1: Observing Bacteria Microbiology Abstract: This lab exercise familiarized the student with the use of a microscope by observing and identifying various different slides under the microscope. The student practiced observing the given slides under the 10x‚ 40x‚ and 100x (oil immersion) objective lenses‚ which allowed for the identification of the different organism’s shapes and sizes. Purpose: The aim of this exercise is to equip the student with the knowledge
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References: Koneman‚ E. W. (1997). Koneman ’s Color Atlas and Textbook of Diagnostic Microbiology. USA: Lippincott Williams & Wilkins. Madigan‚ et. al (2014). Brock Biology of Microorganisms (14th ed.‚ p. 78). Boston: Pearson. Mahon‚ C.‚ Lehman‚ D.‚ & Manuselis‚ G. (2011). Textbook of Diagnostic Microbiology. Missouri: Elsevier. Tille‚ P. M. (2014). Bailey & Scott ’s Diagnostic Microbiology. Missouri: Elsevier.
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Identifying an Unknown Aldehyde or Ketone Introduction The purpose of this lab is to identify an unknown aldehyde or ketone substance using chemical tests. The chemical tests used in this experiment are solubility‚ Schiff‚ Bisulfite‚ and Iodoform tests. Also‚ a 2‚4-dinitrophenylhydrazone derivative synthesis reaction will be completed from which a melting point will be obtained. The chemical test results and the melting point analysis will be compared to the table of compounds given to find the
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It Smells Like Proteus vulgaris Microbiology Lab Report for Unknown Robert Bhowanidin MCB 2010L / Section 1290 October 24th‚ 2013 The following report will describe both my journey to find my unknown as well as the results that led to my discovery. Before I start‚ I will say that I am 100% positive that my unknown (which was #31) is none other than Proteus vulgaris. My data and the ensuing results from them simply cannot be disputed. From my first batch of results‚ Proteus vulgaris reared
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performed this test‚ the initially slant for unknown microorganism #17 is important. For this lab‚ two identical slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants
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Introduction The purpose of this lab was to explore the properties of an unknown compound. An unknown was given and a cation flame test and anion test was performed to determine the identity of the compound. Once the identity was determined‚ the properties were explored. Experimental To determine the cation of the compound‚ a cation flame test was performed. A bunsen burner was lit until a medium blue flame was burning. The given unknown was scooped onto a nichrome wire loop. The wire was held
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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which organism we had in our unknown mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I
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Unknown 2 Scheme “ Fair game” ions Cations: Na+‚ K+‚ NH4+ ‚ Ca2+‚ Mg(OH2)62+ ‚ Al(OH2)63+ Anions: SO42-‚ HSO4-‚ NO3-‚ OH-‚ Cl-‚ CO32-‚ HCO3- Insoluble Compounds: Ca(OH)2‚ CaSO4•2H2O‚ CaCO3‚ MgCO3‚ Mg(OH)2 I. Describe Sample a. Quick description of sample. Ex: Phase‚ color‚ odor‚ crystalline‚ amorphous‚ gel-like‚ powdery‚ etc. II. Flame Test a. Part 1 i. Orange Flame: Na+ is present. K+ ‚ NH4+ ‚ Ca2+‚ Mg(OH2)62 ‚ Al(OH2)63+ may also be present. ii. Purple Flame: K+ is present (no Na+
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The unknown soda ash from experiment 3 was used‚ to determine the weight for each trial we used the equation of (M of HCl) x (18 ml x 105.99) / (10 x 2 x Na2CO3 ). Which was equal to (0.01472 M) x ((18 mL X 105.99)(10 x 2 X 2.428 % )= 0.6 g. To start we had to rinse the beakers‚ electrode and the stirring bar with diluted water. The sample we needed was weighted to the closest 0.1 mg which we got was 0.3 for the first trial. The sample was transferred to a 250 mL beaker and dissolved in 70 mL of
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