References: 1. Beishir‚ L.: Microbiology in Practice: A Self-Instructional Laboratory Course‚ Fifth Edition. (Harper Collins: New York) 1991. 2. Jawetz‚ Melnick and Adelberg: Medical Microbiology‚ Nineteenth Edition. (Appleton and Lange: Norwalk‚ CT) 1991. 3. Tortora‚ Case and Funke: Microbiology: An Introduction‚ Fourth Edition. (Benjamin Cummings: Redwood City‚ CA) 1992. 4. Zubay: Biochemistry. (Addison Wesley:
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CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive
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K. pneumoniae is a gram negative‚ facultative anaerobic‚ gamma-proteobacteria that can cause pneumonia‚ urinary tract infections‚ cholecystitis‚ and septicemia. (Strand & Shulman 1988) As shown in Table 1.‚ many organisms have developed resistances to commonly used antibiotics. The difference between the standard strain of Klebsiella pneumoniae and the extended spectrum betalactemase (ESBL) strain‚ for example‚ is striking. 12 antibiotics had greater than or equal to 95 percent effectiveness against
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disease to knowing the correct microorganism in order to make antibiotics. This study was done by applying the following methods; OF Glucose‚ Indole Production‚ and Malonate Utilization test for the identification of an unknown bacterium. The methods will assist in determining the unknown bacterium found in a 55 year old male that was passing blood and mucous in his stool. The patient had no pain but mild discomfort during bowel movements. The doctor noted inflammation of the lining of the rectum. The
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(2005). Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK. Mol Microbiol 58: Pages 596–612 Gordon‚ G.S Grainge I. (2010). FtsK – a bacterial cell division checkpoint? Molecular Microbiology Volume 78‚ Issue 5‚ pages 1055–1057 Grainge I.‚ Lesterlin C Griffiths A. J. F.‚ Wessler S. R.‚ Lewontin R. C. and Carroll S. B. (2008). Introduction to Genetic Analysis. W.H. Freeman and COmpany. Chapter 7: DNA: Structure and Replication. pages
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DENSITY AND SPECIFIC GRAVITY Materials and Methods Part 1: Density of an Unknown Solid 1. We first were asked from our laboratory instructor to attain an unknown solid and were asked to note down the number of the solid. 2. Determine the mass of the unknown solid to the nearest 0.001 g and record it in the data table in the back of the laboratory workbook. 3. Then we determined the volume of the unknown solid by water displacement. We first filled a 100mL graduated cylinder about
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Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
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colorless colonies. Multiple medical experiments are credited towards transformation‚ especially ones involving cancer and the transformation of normal cells to neoplastic and cancerous cells. Introduction The general purpose of the Transformation Lab was to observe the difference in bacterial growth under differing media conditions to help understand the process of transformation and how it contributes to the life of living organisms. "Transformation" is what occurs when a cell receives and expresses
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Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests‚ and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests
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Introduction: During this unknown lab report various test were performed to differentiate microbes from each other and to compare metabolic and biochemical process. The gram stain distinguishes between Gram positive and Gram negative bacteria based on the composition of the cell wall. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan
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