Chirag Patel Biology 157 Determining the Effect of Varying Substrate Concentration Question: How does concentration of potatoes affect the amount of oxygen produced with hydrogen peroxide and potatoes (enzymes)? Introduction The enzyme used for this experiment is Catalase. Catalase is inside mostly any living organism which uses oxygen. Its job is to break down hydrogen peroxide‚ into oxygen and water. (Formula) 2H2O2 ---> 2H2O + O2 (lab manual). There are limiting factors which if altered
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LAB 1: What temperature does the enzyme actually work properly in? (Hypothesis) If the temperature is below 40 but above 20‚ then the liver will show bubbles. If the temperature is raised higher than the optimum temperature‚ then an extreme decline in enzyme activity would occur following by the quick denaturing of the enzyme‚ rendering it is permanently useless. Also about 37°C is body temperature. The liver that was at 25°C had a huge amount of bubbles (a 4 on the scale) and the 0°C
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How different concentrations of hydrogen peroxide would affect the activity of catalase? 1. Variables Independent Concentration of hydrogen peroxide Dependent Volume of oxygen gas evolved Control Amount of liver used Location of liver sample obtained from the liver Type of liver used Possible enzymes transferred from hands during making liver homogenate Deterioration of liver Endpoint identification Volume of hydrogen peroxide added Reading on the syringe Room temperature
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three different Substrate Concentrations (3%‚ 1% and 0.3%) as which one was the fastest to react‚ I hypothesized that the reaction would occur the fastest with the 3% hydrogen peroxide (the highest amount of concentration) because the higher the concentration the more faster the reaction occurs and helps produce or break up the enzyme. After testing all three of my substrate concentrations with 10 trials each‚ my data showed (first graph) that the 3% substrate concentration had the fastest average
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Chemistry 2500- Exercise C4 The Emission Spectrum of Atomic Hydrogen Objective The purpose of this lab was to calculate an experimental value for the Rydberg constant and then the ionization energy for the hydrogen atom. These values will be obtained by using a prism spectrograph to measure the wavelength value for a section of the visible line spectrum of atomic hydrogen. Theoretical Background When H+ combines with an electron it forms it’s excited state‚ H. This excited atom
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of the enzyme catalyse‚ an enzyme found in food such as potato and liver. Catalyse is used to remove hydrogen peroxide from cells. The enzyme speeds up the rate of decomposition of hydrogen peroxide into water and oxygen. The reaction is: Catalyse is able to speed up the process because the enzyme lowers the activation energy of the reaction. This means that the free energy required for the reaction to take place is made smaller by the presence of catalyse. In fact‚ catalyse is a particularly
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because it can cause hazard for the humans and possibly to the environment‚ the hydrogen peroxide is now as an alternative to replace formalin for a common treatment for parasites (Pedersen and Flemming‚ 2010). Graslund et al‚ 1 (2003) showed that the hydrogen peroxide acts as chemical that served several purposes such as to eliminate bacteria‚ parasite and also algae in shrimp farming. However‚ the hydrogen peroxide still can be hazardous for the health of farmers (Boyd‚ 1999) and also affect
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Lab 3: Hydrogen Spectrum Abstract When white light is viewed through a diffraction grating‚ we can see each component that makes up the light. However‚ when in an excited state‚ a gaseous element produces bright light of specific wavelengths rather than a continuous spectrum of colors. This phenomenon ultimately lead to the Neils Bohr model of the atom in 1913. Introduction In the middle of the 19th century‚ Robert Bunsen and Gustav Kichoff observed that gases emit spectral lines specific
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decided to do my experiment on chicken livers. It was interesting to do this experiment on chicken livers. The results were that since there is DNA I needed to stir the ingredients slowly and do the experiment as quickly as I can due to the fact that there are enzyme in the cells. I found out that liquid detergent is an active agent which the detergent destroy the cell membrane and also the protein. Abstract I chose to do my experiment on chicken livers to see what the results would be. The
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The Effect of Solute Concentration on the Mass of Apples Introduction Background: This lab involves the testing and measuring of water potential. Water potential (ψ) is the tendency of water to diffuse from one region to another. It measures the free energy available in water to do work. Pure water has a water potential of 0 (kpa--unit?). Water will move from an area of higher water potential to an area of lower water potential. Several factors‚ including temperature‚ pressure (ψp) and solute concentration
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