Spectrophotometry was used in the lab to determine whether non-magnetized zeolite‚ magnetized zeolite‚ or charcoal was the more effective sequestration agent for Procion Red Dye. A calibration curve was created with the known concentrations and the absorbances of the Procion Red Dye dilutions at λmax. The slope of the calibration curve was used to determine the concentration of the analytes. As a result‚ charcoal was shown to be the more effective sequestration agent. Introduction PAHs‚ polycyclic
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indirect. Indirect titration works by using a pH meter to measure pH after every addition of the titrant. Direct potentiometry determines the potential of a cell by comparing a reference electrode at a constant concentration against the indicator electrode at different analyte concentrations. For the purposes of this experiment‚ indirect potentiometric titration was performed. Std 1 2 3 Weight 0.2158 0.2058 0.202 Final 42.1 42.4 20.3 Initial 20.3 20.9 0 Vol 21.8 21.5 20
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The Spectrophotometer Determination Of Protein Concentrations And The Effects Sodium Dodecyl Sulphate And Triton X-100 Have On Protein Concentration. INTRODUCTION Spectroscopy is used as a collective term for all the analytical techniques based on the interaction of light and matter. Spectrophotometry is one of the branches of spectroscopy where we measure the absorption of light by molecules that are in a gas or vapour state or dissolved molecules/ions (Tombs‚ et.al‚ 1959). Spectroscopy is the
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Experiment 2: Estimation of protein concentration Name: Wong Jiun Hao ID: 00000011136 Cohort: BM114 Module: Biological Science Table of Contents Title 1 Table of Contents 2 Introduction 3 Objectives 4 Procedure 4 Results 5-6 Discussion 7-8 Conclusion 9 References 9 Introduction The concentration of protein in a sample can be obtained by using the Bradford protein assay method. The method requires a spectrophotometer
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SPECTROPHOTOMETRY Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength It involves with the use of a spectrophotometer. A spectrophotometer is a photometer that can measure intensity as a function of the light source wavelength. A spectrophotometer is commonly used for the measurement of transmittance or reflectance of solutions‚ transparent or opaque solids‚ such as polished glass‚ or gases. Principles
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Construction of a Standard Curve for the Determination of Protein Concentration 09/13/12 PCB 3023-007 Results Figure1: Standard curve. This graph was constructed from the fixed amounts of Bovine Serum Albumin (BSA). Varying amounts of BSA at a concentration of .5µ/µl were mixed with the water and 1mL of coomassie dye. The equation y=0.0259x +0.0511 represents the best fit line for a data set taken from µg of protein equals two to ten µg. Optical density (OD) was surveyed using a Mach
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Experiment 2: Absorbance and Spectrophotometry ABSTRACT: This was an investigation into the effects of different wavelengths of light on methylene blue and carmine red on the absorbance value on a spectrophotometer. A spectrophotometer is used to measure light intensity by emitting a single light source through a cuvette of coloured solution. The particles in the solution‚ which are coloured‚ absorb the light depending on how concentrated it is and this produces an electronic reading from
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scientific method(s) to social debate. To Postman’s claim that; while scientists use math to help uncover and describe the nature of nature‚ sociologists use quantification to give precision to their ideas‚ Kornblum would more than likely argue that Postman is essentially describing the same thing and that what he calls “quantification” is a lot more involved than
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Experiment 7a Name: Date: Title: Purity of Aspirin by Spectrophotometry Aim: i. To measure the absorbance of different volumes of sodium salicylate solutions and aspirin with iron chloride ii. To find the concentration of each standard solutions iii. To determine percentage purity of aspirin. Abstract: The mass of acetylsalicylic acid was determined using a analytical balance. Sodium hydroxide (NaOH) was added to the acetylsalicylic acid and heated in order to hydrolyze acetylsalicylic
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Spectrophotometry. Spectroscopy‚ and the Spectrometer Spectroscopy is the branch of science concerned with the investigation and measurement spectra produced when mater interacts with or emits electromagnetic radiation Meanwhile‚ spectrophotometry is the measurement of color in a solution by determining the amount of light absorbed in the ultraviolet‚ infrared‚ or visible spectrum. A spectrophotometer is an instrument often used to compare the intensity of light from a regulated or standard
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