Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification. B. Introduction I. Fast Protein Liquid Chromatography
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Caffeine is a drug commonly used as a central nervous system stimulant. It is found in plants like coffee and tea. Caffeine can also be found in soft drinks‚ and it is regarded as the most widely consumed stimulant drug in the world based on Nehlig‚ Daval‚ & Debry (1992) research. The interaction of caffeine with adenosine receptors in the brains makes an individual more alert. A moderate intake of caffeine results in small healthy risks while high doses could be a catalyst to negative effects like
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In his book Caffeine Blues a famous researcher‚ health educator‚ and clinical nutritionist Stephen Cherniske compares remaining healthy and strong throughout life to a battle. “Caffeine is the Trojan horse. It looks like a gift but instead delivers adrenalin stress‚ low blood sugar‚ mood and energy swings‚ fatigue‚ depression‚ malnutrition‚ and disturbed sleep”. Although caffeine is considered a mild drug‚ the consequences of caffeine addiction are rather severe and include physical and mental health
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The Caffeine War can be defined as the rush to produce higher concentrations of caffeine to a captive audience‚ by the world’s producers of caffeine products. While there may be many forms of introducing caffeine to customers this report will focus on and compare the Coffee industry and the producers of Energy Drink products while attempting to define their impact and any resulting long term medical harm to the consumer. According to Dr. Howard Axe‚ President of the Chicago Medical society‚ his research
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EXTRACTION Organic Chemistry Laboratory This experiment was conducted in order to compare single and multiple extraction of caffeine from dried tea leaves and to calculate the percentage yield of caffeine for both extraction procedures. The group was assigned to do the multiple extraction. Before the extraction proper‚ the water bath containing the anhydrous sodium carbonate and distilled water was heated. As the sodium carbonate dissolves‚ tea leaves were added in the mixture and allowed to
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Caffeine and Coffee Consumption Maddie Schierbeek Psychology 7th block Why do so many people‚ especially high school students consume so much caffeine? Perhaps 70-minute classes are too much to handle. Maybe the AP course load is overwhelming. Or perhaps problems at home cause a lack of sleep. Everyone has their reasons. On the other hand‚ there may be a scientific reasoning behind it. Caffeine highs are followed by caffeine lows. This cycle results in a need for caffeine: addiction. Researchers
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Introduction ESTERS are compounds derived from the reaction of a organic acid with an alcohol. Acid + Phenol/alcohol --> ester + water R--C=O + R’--OH ----> R--C=O + H2O | | C-OH C-O-R’ Esters are the compounds that give fruits their characteristic flavours and odours. ie. methyl salycilate is "Oil of Wintergreen". http://www.petrik.com/PUBLIC/library/misc/glossary_of_org_chem.htm#ESTERS Aim To synthesise the ester 3-methylbutyl ethanoate( isopentyl acetate) and to identify the product
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The effects of caffeine‚ alcohol and environment on Daphnia Magna. ABSTRACT In this experiment daphnia magna a semitransparent fresh water crustacean‚ are used to study the effects of water‚ alcohol and caffeine on heart rate. Daphnia was placed on a clean deep well slide. This was placed under the microscope and brought into focus on the best objective and light contrast. The heart rate was observed and counted within 15seconds and recorded on a table. Introduction Daphnia magna
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The Separation and Purification of Organic Compounds Sunshine Marie Introduction Each year‚ many thousands of new or unknown organic compounds are prepared or extracted from natural sources such as plants‚ fungi and animals. In order to identify an unknown organic compound or the components of a mixture of organic compounds‚ it is necessary firstly to separate and purify the compounds. At any given temperature‚ a certain number of molecules of a substance in the liquid state have sufficient
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Computing Caffeine Informative Speech John Cedrick D. Balaguer BSIT 3 English 3 : Speech and Oral Communication Skills 1st Semester S.Y. 2013 – 2014 July 17‚ 2014 1 of 1 Caffeine has been used widely around the world over centuries‚ and today it is estimated that 80% of the world’s adult population consumes caffeine in one form or another. What is it about caffeine that has made it so popular? To begin with‚ one of the first characteristic effects of caffeine is its ability
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