Introduction Chitobiase‚ from Vibrio harveyi‚ is a membrane bound lipoprotein involved in the degradation of chitin. Chitobiase is similar to and may share a common ancestry to the a-chain of human b-hexos-aminidase. Chitobiase is encoded by chb. In this experiment‚ a restriction map for restriction enzymes Eco R1‚ Pst1 and Hind III using Southern hybridization and restriction analysis of pRSG 192. pRSG 192 is a recombinant plasmid derived from the chb gene and pUC 19‚ a 2.7kb engineered
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QUESTIONS 1. What are other sources of glycogen? Of starch? a. Other sources of glycogen- Aside from the liver‚ it can also be found in skeletal muscles (for energy during strenuous exercise). It also occurs in tissues including adipose tissues‚ heart muscles‚ kidneys and the brain. It is also found in yeast‚ bacteria‚ fungi‚ molds and algae‚ oysters‚ and shellfish. b. Other sources of starch- Foods that are high in starch include breads‚ grains‚ cereals‚ pasta‚ rice‚ potatoes‚ peas
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Summary of Carbohydrates: Monosaccharides‚ Disaccharides‚ Polysaccharides Carbohydrates are molecules that are synthesized from carbon‚ oxygen‚ and hydrogen atoms. Some types of carbohydrates consist of a single unit consisting of a few atoms‚ while other carbohydrates consists of thousands of units linked together through chemical bonds. Glucose‚ maltose‚ and glycogen are three carbohydrates that are similar‚ but structurally different. Carbohydrates have the general molecular formula CH2O. Starch
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2.4 Utilization of polysaccharide degradation products 2.4.1 Chitin degradation products Chitin can be degraded by chitinase which have the ability to degrade chitin directly to low molecular weight chitooligomers‚ which serve a broad range of industrial‚ agricultural‚ and medical functions. It also has a wide-ranging applications including the preparation of pharmaceutically important chitooligosaccharides and N-acetyl-D-glucoseamine‚ and as mosquito control. Chitooligosaccharides have an extensive
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DEVELOPING A CONVENIENT ANALYTICAL TECHNIQUE FOR THE QUANTITATIVE ESTIMATION OF RESIDUAL CTAB PRESENT IN HEAMOPHILUS INFUENZAE type b POLYSACCHARIDE Thesis submitted for the degree of Bachelor of Technology Biotechnology 1 BY 2 Mr. Sarthak Ranka 2 Under the guidance of 1 Dr. SURESH BERI ADDITIONAL DIRECTOR (PRODUCTION) Serum Institute Of India Ltd 212/2‚ Hadapsar Pune (MH)-411028 1 Submitted to
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are groups of sugars that contain carbon‚ oxygen‚ and hydrogen in a 1:2:1 ratio. Three main units of carbohydrates are monosaccharides‚ disaccharides‚ and polysaccharides. Monosaccharides are a single chain of CH2O while disaccharides are two units bonded together through dehydration reaction but they are both considered sugars. Polysaccharides on the other hand are a combination of three or more units of monosaccharides put together. Carbohydrates are formed by the combination of carbon dioxide and
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observation. (1 mark) (iii) Monomers X can undergo process B to form polysaccharide P. This polysaccharide can exist in two forms as shown in Figure 1.2. [pic] Figure 1.2 (A) Identify polysaccharide P. (1 mark) (B) Suggest ONE function of polysaccharide P. (1 mark) (C) Compare polysaccharide P and cellulose in terms of their structure. Copy Table 1.1 and write the answers
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Pre-lab 1: Identifying Carbohydrates Abstract The point of this lab is to determine if a substance contains carbohydrates such as a reducing sugar and/or polysaccharides. This will be done by using Benedict’s reagent and Iodine stain tests. Benedict’s reagent will react to reducing substances in the solution by oxidizing it and changing the structure of the reducing sugar to form a colored precipitate. The color of this precipitate can be used to determine the concentration of reducing sugars in
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whilst a polypeptide is a chain of amino acids. b) Fat and oil differences: They are both lipids‚ but fats are solid whilst oil are liquids. c) Difference between starch and glycogen: Starch is a polysaccharide found in plant tissue whilst glycogen has polysaccharide found in animals. d) Condensation and hydrolysis: Condensation is a chemical process by which 2 molecules are joined together to make a larger‚ more complex‚ molecule‚ with the loss of water. Instead‚ hydrolysis
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Carbohydrate Polymers 39 (1999) 109–117 Compositional features of polysaccharides from Aloe vera (Aloe barbadensis Miller) plant tissues ´ ´ Antoni Femenia*‚ Emma S. Sanchez‚ Susana Simal‚ Carmen Rossello ´ ´ Enginyeria Quımica‚ Departament de Quımica. Universitat de les Illes Balears‚ Ctra. Valldemossa km 7.5‚ 07071 Palma de Mallorca‚ Illes Balears‚ Spain Received 22 September 1998; received in revised form 27 October 1998; accepted 20 November 1998 Abstract A complete chemical characterisation
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