Column and Thin Layer Chromatography Beverly Abstract: Plant pigments were separated and concentrated from a crude spinach extract through the use of column chromatography and an eluatropic series of hexanes‚ hexane/acetone‚ and methanol. The pigments were analyzed using thin layer chromatography with a 30% ethyl acetate/hexane developing solvent. Introduction: Chromatography is a technique used to separate a mixture of two or more components based on
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Liquid Chromatography – Laboratory #18 Introduction: We are using liquid chromatography to separate the colored substances in grape-flavored drinks. We separate the component dyes‚ and then we separate the flavorings and citric acids. Background: Chromatography is a process that is used to separate a substance into its component parts. The separation occurs between the stationary and moving phase of the lab. The moving phase consists of a fluid and the stationary phase consists of a solid
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Chapter 7 and 8 discuss and give in depth details on photosynthesis and cellular respiration. They are the exact opposites in what they set out to complete in the cells of a leaf. Photosynthesis takes light energy and separates it in the chloroplasts to create glucose. Cellular respiration breaks down glucose to provide energy for the cell. The similarities between them include overlap in some of the enzymes and products and reactants utilized. The differences include the cycles and steps taken to
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com Liquid Chromatography AP Chemistry Laboratory # 18 Publication No. 10535A Catalog No. AP9093 Introduction In this experiment‚ liquid chromatography is used to separate the substances that are present in grape-flavored Kool-Aid®. First‚ the dyes responsible for the purple color‚ FD&C Blue #1 and Red #40 are separated. Then‚ in a second experiment‚ the other components of Kool-Aid®‚ the flavorings and citric acid‚ are separated as well. Concepts • Resolution • Liquid chromatography • Selectivity
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OBJECTIVE The purpose of this experiment is to analyze mixtures of compounds prior to‚ during and after a separation scheme. This experiment also allows monitoring reactions of organic molecules‚ and determines the identity of a mixture of compounds. STRUCTURES AND PHYSICAL PROPERTIES OF REACTANTS [1] SOLVENTS | a. Hexane1Molecular Molarity: 86.18 g/molBoiling Point: 69 ºCMelting Point: -95ºCDensity: 0.659 g/mL at 25ºCWater Solubility: Insoluble in waterColor/Texture: Colorless/LiquidHazardous
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is called chromatography. The materials used consisted of – 3 pieces of chromatography paper‚ a tall clear glass‚ water‚ table salt‚ 1 tsp. measuring spoon‚ an 8 oz. measuring cup‚ pencil‚ ruler‚ 6 washable markers‚ and paper towels. The markers used in this experiment have to be water soluble therefore I used washable markers made by Crayola. The colors I chose were brown‚ teal‚ red‚ orange‚ black‚ and green. The salt solution served as the mobile phase while the chromatography paper served as
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The purpose of this lab was to use gas chromatography to determine the relative amounts of the three components of gas products from the dehydration reaction of 1- and 2-Butanol with sulfuric acid and the dehydrobromination reaction of 1- and 2-Bromobutane with potassium tert-butoxide. E1 reactions are two-step unimolecular reactions. The first step of an E1 reaction occurs when a leaving group‚ which consists of a weak base‚ leaves an organic compound‚ leaving an intermediate carbocation. In the
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measured in the number of bubbles produced by the plant. Three factors that influence the rate of photosynthesis can be adjusted in the simulator to determine how each of the factors affects the rate of photosynthesis. Virtual Lab PreLab Questions Q: To survive‚ what gas do we need to breathe in? __________________________________ Q: Where is this gas produced? _______________________________ A byproduct of photosynthesis in oxygen gas. Plant use some of the oxygen that they produce‚ but most of
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Elodea & Photosynthesis Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize nutrients from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a by-product. Introduction This lab has been created in order to find what extent does distance from a light source (5cm‚ 10cm‚ and 15cm) affect the rate of photosynthesis (measured in bubbles / 3 min) in Elodea water plants. Hypothesis:
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Photosynthesis and Cellular Respiration Nina R. McCray May 5‚ 2015 Biology Lab (SCIE207-1502-A-07) LaDonna McCowan-Ferrier Photosynthesis and Cellular Respiration Lab Report Introduction This is an investigation of the effect of the intensity of light (photosynthesis) have on the rate of cellular respiration (measured as the number of oxygen bubbles). We will determine whether or not it is possible to examine the relationship between photosynthesis and cellular respiration under controlled
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