Abstract Photosynthesis is a food making process for algae and plants. The photosynthesis process rate varies from different wavelengths and intensities of light. This lab will evaluate the optimal wavelengths and degrees of intensity during photosynthesis when chloroplast is exposed to light. The mixtures of DCPIP with water‚ PO4 buffer‚ and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate
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Submit your completed lab report to the Lab: Photosynthesis Lab assignment link for grading. For information on how this assignment will be graded‚ please visit the Course Information sectionChlorophyll and Accessory Pigments A pigment is any substance that absorbs light. The color we see comes from the wavelengths of light that reflect. Chlorophyll‚ the green pigment common to all photosynthetic cells‚ absorbs all wavelengths of visible light except green. The green reflects back to our eyes
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Photosynthesis Lab Data Collection: The absorption of different wavelengths of light by Chlorophyll Wavelength (nm) Absorbance of light by chlorophyll (Arbitrary units) Diluted Calculation New Reading (Arbitrary units) Violet 430 2.35 50% 2.35 x 2 3.20 Blue 470 1.09 - - 1.09 Blue-Green 492 0.38 - - 0.38 Green 520 0.77 - - 0.77 Yellow-Green 550 0.85 - - 0.85 Yellow 580 1.43 - - 1.43 Orange 600 0.65 - - 0.65 Red 700 0.16 - - 0.16 Absorption and reflection of light: Different
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Introduction Photosynthesis is a redox reaction which requires carbon dioxide‚ water and light to produce water and a 6-carbon sugar. The process of photosynthesis consists of two parts‚ a light reaction and a light-independent reaction. The method of changing light energy into chemical energy for the formation of NADPH and ATP is done through the light reactions. Light independent reactions use carbon dioxide and the products of light reactions (ATP and NADPH) to form compounds such as glucose
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The rate of photosynthesis in each of the beakers will depend what is wrapped around the beakers such as red and blue cellophane‚ as well as the control of the experiment the beaker without cellophane. The cellophane will directly impact the light getting absorbed as well as the amount of CO2 in the beakers. Which overall as result will determine the final color of the water mixed with bromothymol blue. What was being measured was the color of the water after CO2 has been blown in the beakers. The
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Bio Block B Photosynthesis Lab 12/6/12 Introduction: Photosynthesis is affected by light intensity‚ water‚ and temperature. Plants grow more abundantly because the weather is warm. Carbon Dioxide given off by animals is consumed by plants that replace the oxygen animals take it. Experimentation will help understanding how plants are vital because of the oxygen they release. If leaf disks in the experiment release oxygen‚ they will undergo photosynthesis and float. If there
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Introduction The purpose of this lab is to determine which pigments in a plant support or effect photosynthesis‚ based on starch production‚ which wavelengths of light are involved in photosynthesis‚ and identify plant pigments found in a plant leaf by means of paper chromatography. Life on Earth is dependent entirely on the energy from the Sun‚ not only to keep the planet at a suitable temperature but also to provide the energy required to sustain life. The energy of the Sun‚ in the form of
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Introduction: Photosynthesis is a process to which some autotrophs such as plants produce their own food. It has two stages or reactions light-dependent and light-dependent reactions. The light dependent reactions are the first stage‚ where energy from sunlight is captures in Photosystem 2 and then 1‚ the electrons generated in Photosystem 1 then moves along the electron transport chain. The moving of electrons causes a hydrogen ion gradient that is used in the final step to produce ATP‚ by the
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rate of photosynthesis until optimal levels are reached‚ then plateauing of results will occur. The hypothesis was refuted by evidence shown in the results (table two) as 0.5% concentration produced the greatest rate of photosynthesis‚ 3.10x10-3. Concentrations of 5% and 10% were deemed unusable as the agapanthus discs would not submerge‚ therefore the results did not enable a range of data. The results of the data indicate that 0.5% NaHCO3 is optimal in determining the rate of photosynthesis‚ 3.10x10-3
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The overall experiment consisted of three smaller experiments as a result of the different properties chlorophylls possess and the need to test them individually to receive the most accurate results. Preparation for the lab began with selecting a medium to large spinach leaf and deveining it. Once the spinach was prepared‚ it was ground in 5 mL of 100% acetone‚ creating a green acetone extract. The extract was filtered through a funnel with a kimwipe and into a test tube. The volume of the filtered
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