166-0003EDU Week 7: pGLO Transformation Introduction to Transformation In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece of DNA which provides the instructions for making (codes for) a protein. This protein gives an organism a particular trait. Genetic transformation literally means change caused by genes‚ and involves the insertion of a gene into an organism in order to change the organism’s trait. Genetic transformation is used in many areas
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Introduction In this week’s laboratory period students had the opportunity to perform a common procedure preformed by many if not all microbiologists known as genetic transformation. Genetic transformation is the ability to move DNA into an organism and thereby altering its genotypic and genetic makeup (2). Genetic transformation has shown to have a wide variety of uses in many scientific studies. In agriculture‚ gene coding for traits such as frost‚ pest‚ or spoilage resistance have been genetically
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Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab‚ the Green Fluorescent Protein‚ which is typically found in the bioluminescent jellyfish Aequorea
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Miguel Felix pGLO Transformation Mr. Betz AP Biology 14 December 2012 Abstract The purpose of this experiment is to determine the effects of the addition of a plasmid to a bacterial cell. The bacteria E. Coli was separated into two groups: one where the pGLO plasmid was added to the bacteria‚ which contains the genes of fluorescence and resistance to antibiotics‚ and the other lacking the plasmid. The two groups then placed in agar plates simulating different environments: the bacteria lacking
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Malak Zomrawi 4/9/15 Bacterial Transformation I. Abstract In the lab‚ the purpose is to see if we could move genes using plasmid. As well as getting better understand of transformation methods using shock wave. To see the effects five trays are being used containing LB nutrient broth. The results showed that the LB‚ ampicillin‚ and arabinose with a positive pGLO had the most amount of growth compared to the other four trays. Although when there is arabinose there is no fluorescence‚ fluorescence
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In this lab‚ we performed a genetic transformation through the process of gene transfer. Gene transfer involves the insertion of a gene into an organism. The gene to be inserted is usually contained in a plasmid‚ which is relatively small‚ circular non-chromosomal DNA molecule typically found in bacteria. Once the plasmid containing the gene is inserted into the organism‚ it is absorbed into the organism’s own genetic code. After this occurs‚ the newly introduced gene begins coding for proteins‚
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Transformation Lab Report Introduction Transformation is the transfers of virulence from one cell to another‚ through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith‚ a British microbiologist. Griffith observed that the mutant form‚ non-virulent form‚ of the bacteria Streptococcus Pnumoniae could be transformed into the normal‚ virulent form‚ when injected into mice along with heat killed normal forms. He concluded that somehow
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Genetic transformation happens when an organism is altered by the introduction of new genetic information which is merged into the organism’s genome. Bacterial transformation is a type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia
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Transformation Of Escherichia Coli With pGLO Plasmid April 24‚ 2013 ABSTRACT: This experiment focuses on genetic engineering and transformation of bacteria. The characteristics of bacteria are altered from an external source to allow them to express a new trait‚ in this case antibiotic resistance. In is experiment foreign DNA is inserted into Escherichia coli in order to alter its phenotype. The goal of the experiment is to transform E. coli with pGLO plasmid‚ which carries a gene for ampicillin
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Genetic Transformation of Bacteria Abstract The transformation of bacteria was successfully carried out using a plasmid carrying a gene that codes for green fluorescent protein‚ which gives a signature green glow reminiscent of a jellyfish. This gene‚ however‚ is only active when the sugar arabinose is present. A gene coding for antibiotic resistance was also found within the plasmid and served as a means to verify that transformation had indeed taken place. The hypothesis was that the bacteria
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