5-35oC‚ with the optimum depending on their normal environmental temperature. The fungi Aspergillus oryzae is heterotrophic which means they taken in their food from dead organic matter and cannot produce food for themselves and use the organic carbon in dead organic matter to grow. Temperature can influence shape of the enzyme within the fungi‚ which effects growth. Enzymes tend to have an optimum temperature for enzyme activity; however it structure can be effected if not within optimum temperature
Free Agar plate Petri dish Fungus
body: Lysosomal lipase‚ hepatic lipase‚ endothelial lipase‚ pancreatic lipase‚ gastric lipase. People with pancreatic shortage and cystic fibrosis often need supplemental lipase and other enzymes. Phenolphthalein C20H14O4 Phenolphthalein is a pH and is often used in titrations; it turns from colourless in acidic
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6 primary functions of digestive system: 1. Ingestion- food intake 2. Secretion 3. Digestion- mechanical & chemical breakdown of food Mechanical Mastication- first step. Enables mixing with saliva to form bolus to be swallowed. Regulated by CNS. Deglutition Mixing/Churning Peristalsis- movement of muscles within GI tract that facilitates movement of food Chemical- Hydrolysis (using H2O) of macromolecules into monomers (residues) carried out by digestive enzymes produced by salivary glands
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The TSA plate was tested for α-amylase production but flooding the plate with iodine and a clear zone did not form around the streak‚ meaning that the organism could not use the starch and therefore no starch utilization. The Pseudomonas-F agar was viewed in the dark under UV light and fluoresced a yellow pigment‚ meaning production of pyoverdin and a positive on fluorescent
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clean” and “Magic power”. Principal: Amalyse can catalyse the breakdown of starch into maltose. In this practical‚ solutions of the 2 washing powders will be filled into 2 identical wells on the starch agar plate separately. Starch will be broken down by the amylase disused to the star-agar. A clean zone will be formed around the wells when iodine solution is added and flushed. The higher the amylase activity‚ the more the starch will be broken down. Hence‚ a larger and clearer zone will be observed
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the presence and the activity of them in the serum is relatively easy‚ comparing to other types of substrates‚ means those enzymes can serve as markers‚ as the concentration of them is increased in the serum indicating tissue damage. For example‚ amylase detected in the serum is a marker for damaged pancreas tissue. The problem arises when the enzyme detected in the serum is present in more than one type of tissue throughout the body. One common example is phosphatase‚ which can be found throughout
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fungal and bacterial amylase was measured as well. Discovering information‚ such as the optimal temperature‚ can help demonstrate the environment these organisms work best. Thus‚ if further studies are conducted on different enzymes‚ the more information is covered about certain organisms. In the experiment‚ each enzyme source was put to the test by being placed into different test tubes and baths with altered temperatures. In order to see how long the Amylase was hydrolyzing starch in different temperatures
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A. Fermentation Lab- The basic process Prepared 3 beakers with contents listed below. ( a. Beaker 1: glucose only b. Beaker 2: Starch only c. Beaker 3: Starch + amylase). Poured contents of each beaker into its respective fermentation tube‚ ensuring the tail portion of the tube was filled with liquid. Placed tubes in an incubator at 37 degrees‚ measuring distance between tip of tube tail to fluid level at 20‚ 40‚ and 60 minute intervals. Calculated gas volume using this distance along with radius
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that is being studied is the hydrolysis of starch. The enzyme that is being studied is amylase. This experiment is looking at the effect of temperature on the rate of the enzymatic hydrolysis of starch. My hypothesis is that the higher the temperature the faster the hydrolysis of the starch would occur. The rationale behind this is that heat is a form of energy and would increase the reaction and speed up the reaction time. It would also cause the starch to hydrolyze before cooler temperatures. Method
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transport proteins (SGLT) in membrane help absorb glucose & galactose Fructose absorbed by facilitated diffusion then converted to glucose inside the cell Protein Digestion & Absorption Pepsin has optimal pH of 1.5 to 3.5 -- inactivated when passes into duodenum & mixes with alkaline pancreatic juice (pH 8) Pancreatic enzymes take over protein digestion by hydrolyzing polypeptides into shorter oligopeptides Brush border enzymes finish the task producing amino acids that are absorbed into the intestinal
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