In the first part of this lab‚ E.coli cells were transformed with an R-plasmid carrying a tetracycline resistant gene‚ giving rise to tetracycline resistant E.coli strain. This was accomplished through transformation‚ which allowed E.coli to directly uptake the naked DNA molecule carrying the antibiotic resistant gene (1). However‚ in order to take up the DNA and incorporate them into their genome via recombination‚ cells must be competent (1). Therefore‚ E.coli cells which are not competent under
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better option than Renting Amanda Newsome University Composition and Communications II November 5‚ 2010 Louisa Fordyce Axia College of University of Phoenix Buying a house is a better long-term investment into the future than renting an apartment. Being mortgage free eventually and having no obligations to pay. “Anyone trapped in a long lease would be wise to consider purchasing property (Cabinet Maker‚ 1998)”. When buying a home it comes with the property it surrounds. After time having
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Renting an apartment is a better option than buying a house I. Introduction Many people may think that renting an apartment is a better option than buying a house‚ but there are many things to take into consideration before deciding. If one were to know the advantages of buying a house‚ one just might change his or her mind. Some people think that renting an apartment is a better option than buying a house because they do not have to worry about the upkeep of the property or have to pay for
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These two English Translations of the Russian poem by Andrei Voznesenky are about a girl being rejected for the first time. The young girl’s feelings of hurt and rejection are described in terms of coldness throughout the poem. “The first ice of human hurt”. The theme that is put across from the poem is of growing up‚ and the experience of loosing someone for the first time. Both of these poems are very similar‚ but they have both been translated in two different styles. This helps to alter the meaning
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spend hours picking out their first day of school outfit‚ and cheer out wrong names of players at football games. While I find all this amusing‚ I also totally remember the excitement‚ anxiousness and remarkable amount of cluelessness that comes with being a brand spanking new college freshman. My first semester of college was certainly an experience. And I use the word experience BECAUSE Experience is what I get when I didn’t get what I wanted. The first semester of my freshman year of college was
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“Yes‚”I thought.The day was finally here! “Do we have everything?” my mother asked. “I think so‚”my father replied. Today was the day we were heading to Washington‚ D.C. “This is my first trip‚” I thought‚ “I hope that nothing happens to our flight”. My mom and my brother were also coming‚ but I wish my brother‚ Myles didn’t have to come. The reason we’re going to Washington‚ D.C. is because my sister‚ Lolo goes to school there. We had to go through security and a bunch of other boring stuff.
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The first and last Dan Savage‚ an American writer‚ once stated‚ “Ultimately life is disease‚ death and oblivion. It’s still better than high school” (Savage). This quote represents what my high school career was like; for example‚ I have been to 4 different high schools. I was in foster care‚ so I would move around a fair amount. Moving around‚ I experienced many first and last experiences in high school. Despite what Savage stated‚ not all of my first and last experiences in high school were horrible
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Living in an apartment instead of a university dormitory has advantages and disadvantages. Discuss some of the advantages and disadvantages of apartment living and then defend your preference. When a person decides to enter a university away from home‚ he or she must also consider living accommodations. Although most universities offer student dormitories‚ students frequently opt to live in an apartment. While there are many advantages to apartment living‚ there are also many disadvantages. Before
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My first culture shock experience was 4 years ago when I came in to this country. It is very diverse here in America. There are many cultures and people from around the world. In my country the ratio is about ninety percent Turkish and My first culture shock experience was 4 years ago when I came in to this country. It is very diverse here in America. There are many cultures and people from around the world. In my country the ratio is about ninety percent Turkish and theMy first culture shock experience
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anyways. I was pretty nervous. On the first day of school‚ my mom woke me up by turning on my light and said “first day of school! Wake up! I then went downstairs and quickly ate breakfast. Then I raced upstairs to my room to get dressed‚ followed by brushing my teeth. All I could think about was that it’s my first day of middle school. The more and more I thought about it‚ the more butterflies I could feel in my stomach. Before I left‚ I brushed my dark‚ straightened hair‚ and said “bye mom
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