chapter 03 was carried out to identify pathogenic and nonpathogenic bacteria present in the modified spines of C. subspinosus; chapter 04 deals with the development and characterization through next-generation sequencing (NGS)‚ 30 unpublished microsatellite markers‚ polymorphic and specific to the species; and chapter 05‚ developed markers were used as molecular tools to assess level of structure and genetic diversity‚ gene flow‚ allelic composition of C. subspinosus. With biological knowledge obtained
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The external environment’s effects upon genes can influence disease‚ and some of these effects can be inherited in humans. Studies investigating how environmental factors impact the genetics of an individual’s offspring are difficult to design. However‚ in certain parts of the world in which social systems are highly centralized‚ environmental information that might have influenced families can be obtained. For example‚ Swedish scientists recently conducted investigations examining whether nutrition
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Eagle‚ and Loughborough‚ inferred from microsatellite genotypes. Abstract: This study was undertaken in order to compare the heterozygosity of three Lake Trout populations at various loci. Samples of twenty-five Lake Trout were collected from three lakes: Devil‚ Eagle and Loughborough‚ all three of which are situated north of Kingston‚ Ontario. An autoradiograph was used to analyze the genotypes of the individuals at six different loci of microsatellites‚ which are repeat sequences in the DNA
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A comparison of observed heterozygosity of the black rhino and white rhino This study was undertaken in order to understand the heterozygosity trends of the white and black rhinos. Genotypes of five microsatellite loci were collected and assessed with the intention of producing data for a sixth locus from the black and white rhino autoradiograph. This method was a means of analyzing the heterozygosity of the two species of rhino. It was established that black rhinos possess
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Prairie voles are monogamous organisms where both parents are involved in rearing their young. Meadow voles‚ a closely related species‚ are polygamous where the mother is the only parent involved in rearing the young. Lim et al. (2004) determined that dopamine and vasopressin are key hormones in regulating social behaviors. Elevated levels of vasopressin V1a receptor (V1aR) was discovered to cause the differences in behavioral differences between the prairie vole and the meadow vole. Dopamine
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Unfavorable environmental conditions may induce flowering plants to reproduce by clonal growth more frequently than sexual reproduction‚ and such environmental conditions can be temporary or long lasting (HONNAY and BOSSUYT‚ 2005). A plant species can form remnant population when stages of the life-cycle persist over time due the failure to complete the whole life cycle (Eriksson 2000). These remnant populations may play an important ecological role for ecosystem resilience‚ due to positive interactions
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High resolution of human evolutionary trees with polymorphic microsatellite. Nature 368: 455-457. 3. Bradley D G‚ MacHugh D E‚ Cunning-ham P and Loftus R T (1996). Mitochondrial diversity and the origins of African and European cattle. Proceedings of the National Academy of Sciences of the USA 93: 5131-5135. 4. Buchanan F C‚ Adams L J‚ Littlejohn R P (1994) Determination of evolutionary relationships among sheep breeds using microsatellites. Genomics 22: 397-403. 5. CAST (1984). Animal germplasm preservation
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with the reduction of viable grassland habitat‚ leading to fragmentation of populations. For their experiment‚ they took various blood hair and tissue samples from locations across the United States. From their 589 DNA samples they analyzed 15 microsatellite loci amplified using PCR. For a control‚ they used approximately 250 base pairs of mitochondrial
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American Leaf Blight (Microcyclus ulei) in the rubber tree. Theor Appl Genet 100: 975–984 Lespinasse D‚ Rodier-Goud M‚ Grivet L‚ Leconte A‚ Legnate H‚ Seguin M (2000) A saturated genetic linkage map of rubber tree (Hevea spp) based on RFLP‚ AFLP‚ microsatellite and isozyme markers. Ther Appl Genet 100: 127–138 Metcalfe CR (1966) Distribution of latex in the plant kingdom. Econ Bot 21: 115–127 Montoro P‚ Rattana W‚ Pujade-Renaud V‚ Michaux-Ferriere N‚ Monkolsook Y‚ Kanthapura R‚ Adunsadthapong S (2003)
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cross-validation. J Am Stat Assoc 78:316–331. CrossRefWeb of Science ↵ Estoup A and Angers B‚ 1998. Microsatellites and minisatellites for molecular ecology: theoretical and empirical considerations. In: Advances in molecular ecology (Carvalho G‚ ed). Amsterdam: IOS Press; 55–86. ↵ Estoup A‚ Rousset F‚ Michalakis Y‚ Cornuet JM‚ Adriamanga M‚ and Guyomard R‚ 1998. Comparative analysis of microsatellite and allozyme markers: a case study investigating microgeographic differentiation in brown trout
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