Microbiology Final exam Study reference 1. Gram Stain * Verify if bacteria are present or not. * Controls – positive (purple) – S.aureus negative ( red/pink) – E.coli 2. Endospore Stain Positive controller – B. magneterium Green spore- Positive Pink (vegetative ) – Negative 3. Acid fast Positive control – M. smeagmatis Blue – negative Pink /red- positive 4. Motility Positive control - P.vulgaris A. Non motile is negative test B. Motile is a positive test 5. Carbohydrates
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Identifying an Unknown Aldehyde or Ketone Introduction The purpose of this lab is to identify an unknown aldehyde or ketone substance using chemical tests. The chemical tests used in this experiment are solubility‚ Schiff‚ Bisulfite‚ and Iodoform tests. Also‚ a 2‚4-dinitrophenylhydrazone derivative synthesis reaction will be completed from which a melting point will be obtained. The chemical test results and the melting point analysis will be compared to the table of compounds given to find the
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understand this lab we first need to know what gram-negative and gram-positive bacteria are and what it’s composed of. Gram-positive bacteria take up the class of bacteria that stain crystal violet in the method of bacterial differentiation. Where gram-negative bacteria are a class of bacteria that does not retain crystal violet. Instead they are counter-stained pink by Safranin‚ and because of this‚ identifying positive is possible. One of the many differences between gram-negative and gram-positive bacteria
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performed this test‚ the initially slant for unknown microorganism #17 is important. For this lab‚ two identical slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants
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It Smells Like Proteus vulgaris Microbiology Lab Report for Unknown Robert Bhowanidin MCB 2010L / Section 1290 October 24th‚ 2013 The following report will describe both my journey to find my unknown as well as the results that led to my discovery. Before I start‚ I will say that I am 100% positive that my unknown (which was #31) is none other than Proteus vulgaris. My data and the ensuing results from them simply cannot be disputed. From my first batch of results‚ Proteus vulgaris reared
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Identification of Unknown Report Abstract: The purpose of this lab was to determine the identity of an unknown bacteria slant culture. The unknown was identified using differential tests to determine its metabolic and morphology characteristics. Results from the differential tests indicated that unknown culture 5 is Staphylococcus aureus. Introduction: The purpose of this lab was to determine the identity of an unknown bacteria slant culture using a series of differential tests. The tests
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MIC 206 Guidelines for Unknown Investigation Lab Report General Considerations: A. Papers must be typed‚ written in past tense‚ and in third person. (-50 if not typed) B. Paper Structure: Each section is to be headed with the appropriate heading. C. Format and Style 1. Format: a. Face Page: The face page should contain the title of the experiment‚ the author of the report‚ class section time‚ & date submitted. (1.5 points) b.
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_____________________________________________ Project 3: Identifying the Unknown White Compound by Testing Physical and Chemical Properties _____________________________________________ Michigan State University Chemistry Lab 161 Section 27 19 October 2017 Friday 11:00-1:50 Introduction For this experiment‚ there was an unknown white compound that had to be identified. By testing this compound for different properties it made making an educated guess easier to determine
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Requirements for Unknown Report You have been provided a mixed culture (a gram positive and a gram negative) from which you should have isolated each type of organism‚ described their colonies on a TSA plate‚ and described their cellular morphology. You were graded on this activity separately. After you have isolated your gram negative in pure culture you will be subjecting it to various biochemical tests and making observations about the cells‚ colonies‚ growth temperature preferences‚ etc. in
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Unknown 2 Scheme “ Fair game” ions Cations: Na+‚ K+‚ NH4+ ‚ Ca2+‚ Mg(OH2)62+ ‚ Al(OH2)63+ Anions: SO42-‚ HSO4-‚ NO3-‚ OH-‚ Cl-‚ CO32-‚ HCO3- Insoluble Compounds: Ca(OH)2‚ CaSO4•2H2O‚ CaCO3‚ MgCO3‚ Mg(OH)2 I. Describe Sample a. Quick description of sample. Ex: Phase‚ color‚ odor‚ crystalline‚ amorphous‚ gel-like‚ powdery‚ etc. II. Flame Test a. Part 1 i. Orange Flame: Na+ is present. K+ ‚ NH4+ ‚ Ca2+‚ Mg(OH2)62 ‚ Al(OH2)63+ may also be present. ii. Purple Flame: K+ is present (no Na+
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