separate individual cells of a particular microbe. This requires the use of a solid medium that provides a surface for the individual cells to be separated and isolated from the other microbial cells that may be present in the original sample. C. Compare your L. acidophilus pour plates and spread plate. Which method do you think worked better to isolate individual colonies? Why? I think spread plates worked best because a solid medium that provides a surface for the individual cells to
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Isolation of Caffeine from Tea (12A) Introduction When a person drinks tea or coffee‚ it is not pure caffeine. It also has other natural substances. The purpose of this lab was to isolate caffeine from tea leaves. The methylene chloride removed nearly pure caffeine from the basic tea solution. Caffeine is a white‚ crystalline‚ bitter alkaloid‚ C8H10N4O2 ‚ usually derived from coffee or tea. Caffeine is used in medicine chiefly as a nervous system stimulant. Caffeine is used by humans to ward off
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Lab 1: Observation of Chemical Changes Name: Tyler Lee Lab Partners: none Date of Experiment: Feb 26 2015 Location: My House Course Number: CHE111 Abstract: The point of this lab was to test and observe chemical changes when mixing chemicals together and also through heating chemicals. Experiment and Observations: The experiment performed in the first part of this lab was to mix various chemical solutions together and then observe and record the chemical changes‚ also if a chemical
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Lab 2: Infra-Red (IR) - Nuclear Magnetic Resonance (NMR) Exercises In Molecular Spectroscopy - Structural Determination Organic Chemistry II CHEM 2425 Manrique October 30‚ 2012 Alex Wolf Introduction For the purposes of this lab‚ the chemical formula will be given‚ and the degrees of unsaturation can be calculate from the formula (2C+2)-(H+Hal-N)/2. The degrees of unsaturation will give clues as to the structure. A double bond‚ as well as a ring will have one degree of unsaturation each
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Math Practice Lab Pre-Lab Questions: 1. The rules concerning handling significant figures are as follows: When dividing/multiplying The answer has no more significant digits than the number with the fewest significant digits (the least precise figure). Round off after calculations have been performed. When adding/subtracting Answer has no more places than the addend‚ minuend‚ or subtrahend with the fewest number of decimal places. Significant figures are irrelevant when adding/subtracting
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PLASMID DNA ISOLATION‚ RESTRICTION ENZUME DISGESTION AND AGAROSE GEL ELECTRIPHORESIS Abstract: The gel is covered with an ion- containing buffer‚ such as (TAE)‚ that controls the pH of the system and conducts electricity. overall DNA concentration was lower than expected. Using agarose gel electrophoresis is to separate and visualize the DNA fragment‚ which is produced by restriction enzymes . Introduction: The purpose of this experiment is to measure the size of the fragments of DNA and separate
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To My parents‚ Mr. Therdsak and Mrs. Aree Jainonthee who always behind every success of my life ACKNOWLEDGEMENT I would like to express my sincere thanks to my thesis advisor‚ Asst. Prof. Dr. Duangporn Pichpol for her invaluable suggestions and encouragement throughout the period of this research. I am most grateful for her teaching and advice on research methodologies and techniques performed in microbiology testing. Her close attention on my working life and my career path are very much appreciated
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Title: Observations of Chemical Changes Purpose: To learn about the international system of units (SI)‚ to become familiar with common lab equipment and techniques‚ to gain proficiency in determining volume‚ mass‚ length‚ and temperature of a variety of items using common laboratory measurement devices‚ to learn to combine units to determine density and concentration‚ and to use laboratory equipment to create serial dilutions and determine the density and concentration of each dilution. Procedure:
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Bromination Solo Experiment 3 – Individual Lab Report (Save as pdf and submit‚ due by 12:00 NOON one week after experiment) Last Name: First Name: TA Name: Date Lab Performed: Date Lab Submitted: Group A‚ B‚ or C: Comments for Grading TA: (Please indicate if you performed the lab on a day other than your regularly scheduled day and/or with a TA other than your regular TA). Page Limit: report must not exceed FIVE pages (including this page) LIMIT DOES NOT INCLUDE ANY GRAPHS‚ SPECTRA
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Isolation‚ Morphological‚ and Biochemical Characterization. A high-throughput screening of cartenoid-producing microorganisms resulted in 25 astaxanthin-producing bacterial strains from marine water samples that were collected from the Pacific coast of Japan (unpublished results). Among the isolates‚ a novel‚ highly selective astaxanthin-producing marine bacterium‚ labeled strain (N-5)‚ was isolated based on its ability to produce orange pigment on NA plate after 2 days of cultivation at 30 °C. Strain
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