Method • Methodology is performed through 3 successive days: First Day: Trypan Blue cell viability assay: Description Trypan blue staining is a simple way to evaluate cell membrane integrity (and thus assume cell proliferation or death) but the method is not sensitive and cannot be adapted for high through put screening. Short 96 well assay: EACH condition should be done in triplicate or more. Steps 1. Trypsinize one T-25 flask and add 5 ml of complete media to trypsinized cells. Centrifuge
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This reagent can be used to precipitate alkaloids due to the basic in nature of alkaloids. When a few drops of Hager’s reagent were added in the extract‚ a yellow precipitate was formed. Mayer’s reagent (Potassium-Mercuric Iodide) also can used to test the presence of alkaloids. Alkaloids were known to form salt with compounds of mercury. Mayer’s reagent reacts with lone pairs of nitrogen that present in alkaloids to form creamy
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has a specific trait. For i.e. If sugar ferments there will be a color change. III. Reagents: 1.Tryptone 2.Yeast extract 3.NaCl 4.KCl 5.MgCl2 6. MgSO4 7.Glucose/ Dextrose 8.H20 9.Agar( for solid) IV. Methods Grab an Erlenhymer flask for the solid solution and another flask for the liquid solution. Label both flasks. Put 200 ml of distilled water in each flask Then weigh out each of the reagents. Pour the reagents into each flask Swirl/mix the liquid flask. When everything is dissolved cap it off
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stable relationship with the good guy‚ Don Padilla‚ in her corporate “barkada”. Don is a responsible person which is Ellie’s ideal guy. There is this ‘Rock Star’ that Ellie and her friend‚ Charisse call. He’s name wasn’t really Rock Star but Lucas Haresco. Lucas is their officemate and an Office Crush that even Ellie liked. Conflict: Something was wrong with Ellie and Don’s relationship that Ellie noticed but she did not complain. It was Holy Week and Ellie and Don went out for a Visita Iglesia
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The place of unmarried women in British society was determined by their social status and the size of their dowries. Married women had more freedom and influence than unmarried women‚ and their positions were defined by the rank and wealth of their husbands. Unmarried women could be respected and influential‚ only if they were of high birth and had a great deal of money. Women of the low gentry‚ who were unlucky enough to have small dowries (or no dowries at all)‚ were relegated to borderline poverty
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correctly. 1. The substrate for amylase is Your answer : b. starch. Correct answer: e. starch and carbohydrate. 2. Which of the following is true of enzymes? You correctly answered: c. Their activity can be affected by temperature and pH. 3. The reagent IKI tests for the presence of You correctly answered: a. starch. 4. Which of the following is not true of controls? You correctly answered: d. A negative result with a positive control is required to validate the test 5. Which of the following is
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Indigo Synthesis Author: Carolina Morales Group #1 Lab Partners: Jennifer Capeloto‚ Samantha Hoffman and Adriana Garibay Instructor: Saehan Park Chem. 152‚ Section 43 Date Work Performed: February 10‚ 2010 Date Work Submitted: February 24‚ 2010 Abstract: The percent yield of the color Indigo that is the color of blue jeans for the three trials was determined to be 13.50%‚ 15.30% and 14.80%. A series of experiments were performed to a cloth dye comparison. The absorbance
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UNKNOW BACTERIA LAB REPORT UNKNOWN 36 Introduction The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others
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can be made about this experiment? Egg white and pepsin contain proteins. C. How might Biuret reagent be used in a practical real-life situation? To see if a cell or food has proteins in it. D. How did the predicted results compare to the actual results? I was correct on 3 of the 4. I predicted pepsin incorrectly. Exercise 2: Testing for Sugars Observations Data Table 2: Benedict’s Reagent Results. Test Sample Predicted Results Benedict’s results #1 Glucose none positive #2 Starch none
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Scott‚ THX 1138 by George Lucas‚ and Children of Men by Alfonso Cuaron‚ as the landscapes help develop the complexity of the story. The directors of these films use setting and landscape to express and contribute to the development of the main argument of each film. In Blade Runner‚ Ridley Scott immediately constructs a dark‚ gloomy‚ and worn out Los Angeles to convey the main argument that if society is not maintained it will end up in destitution
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