Characterization of Saponifiable Lipids and Characterization & Isolation of Complex Lipids Jan Kevin Roxas‚ Nashiba Samad‚ Erickson Santos Department of Chemistry‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Lipids building blocks of different organic material that are water insoluble. This can be divided into simple and complex lipids that have several forms like waxes‚ triacylglycerols‚ phosphoacylgylcerols‚ sphingolipids‚ eicosanoids‚ isoprenoids and many other. To identify‚
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Lipids Lipids don’t dissolve in water contains mostly C‚H‚O (lower ratio of oxygen than do carbohydrates Types: Triglycerides‚ Waxes‚ Phospholipids‚ Sterols Triglycerides: contain glycerol molecule with three fatty acids attached‚ glycerol forms backbone different triglycerides are formed by various fatty acids that attach. Types of triglycerides: Saturated Fat‚ Unsaturated Fat‚ Monounsaturated Fat‚ and Polyunsaturated Fat Saturated Fat: all carbons are saturated with hydrogens there
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Fats‚ waxes‚ monoglycerides‚ and triglycerides are all types of lipids. Triglycerides are a main form of energy for both plants and animals. Triglycerides are broken down into 3 fatty acid chains. Each fatty acid chain is further broken down into multiple groups of Acetyl CoA. Acetyl CoA molecules go through the citric acid cycle and the electron transport chain. 12 ATP are formed from each Acetyl CoA molecule. Carbon chains that are saturated with hydrogen atoms Chains are straight and
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The Chemistry of Lipids Experiment #8 Objective To observe the solubility of lipids in polar and nonpolar solvents and to compare saturated and unsaturated fats in their chemical reaction with bromine. The percent fat in a food will be determined by extraction of the fat and weighing it. Introduction Lipids‚ by definition‚ are natural substances that do not mix with water but dissolve in organic solvents. There are several classes of lipids‚ including: fatty acids‚ waxes‚ triacylglycerols
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The Biological Importance of Lipids Lipids are organic compounds found in all types of plant and animal cells. They always contain carbon‚ hydrogen and oxygen‚ however the relative amount of oxygen is less than the amount of carbohydrates in lipids. Lipids are polymers and the long chains of repeating units are joined together in a condensation reaction where water is also produced. The most common types of lipids are triglycerides‚ phospholipids and waxes. Triglycerides are formed by three fatty
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=========== BIOCHEMISTRY ============ Biochemistry Laboratory - CH 601L (2011-2012) Experiment 6 Characterization of Saponifiable Lipids Kevin Arven A. Benin*‚ and Gabrielle Marie A. Bugna Department of Chemistry‚ College of Science University of Santo Tomas‚ Espana Street‚ Manila 1050 Date Submitted: August 25‚ 2011 ________________________________________________________________________ Abstract:
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Lipids are made up of carbon‚ hydrogen and oxygen (phospholipids also contain phosphate) and are found in a wide variety of organisms from bacteria to eukaryotes where they perform a wide variety of useful functions. This essay will expand on these functions to describe the role of lipids. There are several types of lipid including triglycerides‚ steroids‚ waxes and phospholipids. Triglycerides are made up of three fatty acids combined with glycerol by an ester linkage meaning they are predominantly
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Metabolism “Metabolism is the set of chemical reactions that happen in living organisms to maintain life. These processes allow organisms to grow and reproduce‚ maintain their structures‚ and respond to their environments” (Metabolism). Metabolism breaks down the food that we eat‚ transforming it into energy for our bodies. Metabolism is broken down into two categories Anabolism and Catabolism‚ which help aid in the chemical reaction process. Specific proteins in the body control the chemical
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Isolation and Characterization of Complex Lipids Shea Kathleen Guinto‚ Peter Julius Dale Inocando*‚ SooHyun Jung‚ Jurilla Camille Louise Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract In this experiment an egg yolk will serve as the sample to isolate lipids and separate them into two classes which is the phosphorylated lipid and the non-phosphorylated lipid. The isolated lipids are then characterized through a series of color chemical tests along with the given
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Metabolism By: Who ever Enzymes Enzymes act as a catalyst by speeding up or slowing down processes in the body. A protein enzyme has a particular shape that contains an active site. An active site is where a substrate fits into place to have its bonds either built or broken. At which time the products or product produced are released. The enzyme remains to continue to act on any available substrate. Hereditary Fructose Intolerance AldolaseB is found mainly in the liver. The second
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