Samples of DNA with the target sequence are heated to a reaction temperature of 94-96˚ C that causes DNA melting of the DNA template without denaturing the enzyme by disrupting the hydrogen bonds between complementary bases of the double helical DNA‚ yielding single-stranded DNA molecules(“How Is PCR (polymerase Chain Reaction) Done?”). DNA polymerase does not get degraded in such high temperatures since the DNA polymerase used in this reaction is thermostable as it is isolated from thermophilic bacteria
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Name: Brian James|Date:3/10/13| Exp 9: Stoichiometry of a Precipitation Reaction|Lab Section: 73426| Data Tables: Step 3: Show the calculation of the needed amount of Na2CO3 CaCl2.H2O(aq)= m/M =1/147 =0.0068 mol CaCO3(s)=0.0068*1/1 =0.0068 mol CaCO3(s)= CaCO3 (s)= CaCO3 mol *CaCO3 g =0.0068 mol*100.01 g =.68 g Step 4: Mass of weighing dish _0.6_g Mass of weighing dish and Na2CO3 .72_g Net mass of the Na2CO3 .12_g Step 6: Mass of filter paper
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.6Name: Danielle Hall|Date: 10.15.2012| Exp 9: Stoichiometry of a Precipitation Reaction|Lab Section: | Data Tables: Step 3: Show the calculation of the needed amount of Na2CO3 CaCl2.H2O(aq)= m/M =1/147 =0.0068 mol CaCO3(s)=0.0068*1/1 =0.0068 mol CaCO3(s)= CaCO3 (s)= CaCO3 mol *CaCO3 g =0.0068 mol*100.01 g =.68 g Step 4: Mass of weighing dish _0.6___g Mass of weighing dish and Na2CO3 __0.72__g Net mass of the Na2CO3 ___0.12_g Step 6: Mass of filter paper
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Lab 7: SN1 and SN2 Reactions Discussion/Conclusion: In this experiment the purpose of the lab was to study the relationship of structure and reactivity in nucleophilic substitution reactions by using qualitative tests of reactivity. Before the experiment began‚ students were asked to predict whether the reagents would cause an SN1 mechanism‚ SN2 mechanism‚ or both. When predicting the outcome of SN1 and SN2 reactions‚ the nature of the carbon skeleton‚ nature of the solvent‚ and the nature of
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REACTION PAPER It is said that effective teacher will use both assessment and evaluation regularly to improve student learning and to guide instruction. Indeed‚ both have pivotal roles in the teaching-learning process yet‚ somehow they do differ in many ways. Assessment and evaluation measure whether or not learning and/or learning objectives are being met. One could look at assessment and evaluation as the journey (assessment) versus the snapshot (evaluation). (Sue Watson‚ 2009) Assessment requires
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Laboratory 9 Lab title: Conductivity of solutions and Household Items PURPOSE: To understand the compounds that dissociate (break into ions) when dissolved in water. Determine ionic compounds are electrolytes. To identify solutions as electrolytic and non-electrolytic‚ and to identify variations in conductivity among electrolytes. Substances that are capable of conducting an electric current in solution are known as electrolytes. Substances that do not conduct an electric current
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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AP Chem Exam - ‘98 1. Solve the following problem related to the solubility equilibria of some metal hydroxides in aqueous solution. (a) The solubility of Cu(OH)2(s) is 1.72 x10–6 g/100. mL of solution at 25° C. (i) Write the balanced chemical equation for the dissociation of Cu(OH)2(s) in aqueous solution. Cu(OH)2 Cu 2+ + 2 OH – (ii) Calculate the solubility (in mol/L) of Cu(OH)2 at 25 °C. (1.72 x10–6 g/0.100 L)(1 mol/97.5 g) = 1.76 x10–7 mol/L (iii) Calculate
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Toxicology Lab 1. In this investigation‚ a wide range of concentrations of Sodium Chloride (NaCl) solution were created and the effects that they had on radish seeds were tested. This ultimately created a doseresponse experiment in which it was detectable whether or not radish seeds were a reliable bioassay for the toxicity of NaCl. The goal of this experiment was to determine a correlation between toxicity and seed germination/radicle
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Observations | Reaction Type | Complete and Balance | 1. When heated in oxygen‚ magnesium gives off a bright white flame. | combination reaction | 2Mg + O2-----> 2MgO | 2. Bubbling and fizzing occurs; the zinc appears to “dissolve” and what appears to be “steam” comes off out of container. | single-displacement reaction | Zn + 2 HCl (aq) --> ZnCl2(aq) + H2(g) | 3. Black smoke occurs | decomposition reactions | 2 (H2O2)(aq) MnO2 2 (H2O) + (O2) | 4. reddish-brown deposit
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