Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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accelerate the rate of a reaction by factors of at least a million as compared to the same reaction without the enzyme. Most biological reaction rates are not perceivable in the absence of the enzyme. The term enzyme was first used by a German pshysiologist Wilhelm Kühne in 1897. There are over 700 different kinds of enzymes that have been identified. Enzymes can be classified into several categories such as hydrolytic‚ oxidizing‚ and reducing. One of the simplest biological reactions by an enzyme is the
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affecting the rate of reaction‚ PNPP (p-nitrophenyl phosphate) + H20 ? PNP (p-nitrophenol) + H3P04. This reaction is catalyzed by the enzyme phosphatase. Different environments produced different reaction rates as environmental factors affect the efficiency of phosphatase. This is because environmental factors can change the tertiary structure of phosphatase‚ which alters its active site‚ and thus changes its efficiency to catalyze the reaction. We measured the rate of reaction‚ by using a chromogenic
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Ted Legdeto Mark Maguire 10/15/13 Period G Observation and Experiment Lab Objective: To make observations about the changes that occur when several substances are mixed and to design controlled experiments to identify the substances responsible for the observed changes. Materials: We did not use graduated cylinders or beakers. Apparatus Expendables Wash bottle Pen for labeling Jars or plastic cups‚ small‚ 2 Sealable‚ zipper-lock plastic bags‚ quart size‚ 6 Measuring spoons
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Enzyme Kinetics Marcos‚ Nelissa S. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City 1101 Philippines ABSTRACT The rationale of the experiment is basically founded in the concept of reaction rates as affected by enzyme‚ and how the enzyme works is competed by a competitive inhibitor‚ thereby impeding the forward reaction. In this experiment‚ o-diphenol oxidase‚ an enzyme that causes the browning in fruits‚ was extracted from banana and reaction rate of this was established
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Kinetic Art Kinetic art: the art of motion. Kinetic art contains a/or several moving parts and depends on motion for its effect. The motion of the art work can be provided in virtually any way. It can be powered by natures elements; wind‚ wave power from water‚ or heat from a fire; It can also be powered mechanically through electricity‚ steam‚ clockwork using earths natural phenomena’s ‚ gravity‚ motors‚ or even through the observer such as cranking a handle‚ or giving a push. (Kinetic) What
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of kinetic constants (Km and Vmax) of extracellular purified pectinase. 3.8.3. Effect of temperature of the reaction: The effect of the temperature of the reaction on the activity of the purified enzyme was carried out by make the enzymatic reaction for 10 minutes at different temperature 25‚30‚35‚40‚45‚50‚60 and 70°C using an enzyme protein 0.1mg/reaction mixture and substrate concentration of 15 mg/reaction mixture‚ using a control of previously heated enzyme solution in the reaction. The
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Chemical Reactions Lab Report Introduction: Chemical reactions is a process that involves rearrangement of the molecular or ionic structure of a substance. There are different types of reaction such synthesis‚ decomposition‚ single replacement‚ double replacement‚ and combustion. Synthesis is where two or more reactants combine to create a product. For Decomposition‚ it is the opposite where a product breaks down into reactants. In Single Replacement‚ reactants switches an element with another element
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Name Lab Partner Name(s) Thermodynamics‚ Enthalpy of Reaction Lab March 27‚ 2014 I. Purpose The purpose of this lab is to learn how to determine the heat of reaction. II. Safety 1. Wear appropriate safety attire (goggles‚ aprons‚ hair tie‚ etc.). 2. Do not directly inhale or ingest chemicals. 3. Understand safety information regarding the chemicals being handled during the lab (hydrochloric acid‚ sodium hydroxide). 4. Be aware of how to operate all lab equipment. 5. Clean hands
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The idea of kinetic art is getting a bit of a workout at the moment. MIT Museum recently hosted “year of kinetic art‚ including “5000 Moving Parts‚” a kinetic art exhibiton featuring large-scale works by Arthur Ganson‚ Anne Lilly‚ Rafael Lozano-Hemmer and John Douglas Powers. Plus the Kinetic Art Organization has published a digital “International Collection of Essays About Kinetic Art—2013—volume 1.” The two don’t overlap: The MIT show highlights a somewhat different segment of artists working
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