BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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Kinetic and Thermodynamic Reaction Condition Brandon Bullock 2/10/15 Objective The purpose of this experiment is to test kinetic theory by running several reactions and determine which product are formed under kinetic conditions and which products are formed under thermodynamic conditions. Kinetically controlled products have low transition states. This makes it easy for the product to be formed at low temperatures
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Enzymes are naturally occurring biological catalysts that are extremely efficient and specific. Enzymes accelerate the rate of a reaction by factors of at least a million as compared to the same reaction without the enzyme. Most biological reaction rates are not perceivable in the absence of the enzyme. The term enzyme was first used by a German pshysiologist Wilhelm Kühne in 1897. There are over 700 different kinds of enzymes that have been identified. Enzymes can be classified into several categories
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Enzyme Kinetics Marcos‚ Nelissa S. Institute of Chemistry‚ University of the Philippines‚ Diliman‚ Quezon City 1101 Philippines ABSTRACT The rationale of the experiment is basically founded in the concept of reaction rates as affected by enzyme‚ and how the enzyme works is competed by a competitive inhibitor‚ thereby impeding the forward reaction. In this experiment‚ o-diphenol oxidase‚ an enzyme that causes the browning in fruits‚ was extracted from banana and reaction rate of this was established
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Achievement Standard 91106 (2.9) Log number: 4 Title: The Heat Text type: Film Director: Paul Feig The film ‘The Heat’ is a film about a very formal but cocky FBI agent who is paired with a really aggressive Boston cop to take down a ruthless drug lord. The FBI agent‚ Sarah Ashburn‚ only agreed to take on this case to get a promotion. The Boston cop‚ Mullins‚ was chosen to do this case because she knew the town better than anyone else did. Sarah Ashburn and Mullins did not like each other
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F7 Essay Writing (Kinetics) Q. Write an essay on factors which affect the rate of reactions and discuss the uses of kinetic studies. Outlines: (I) Factors affecting the Rate of Reaction (a) temperature ---- collision of molecules with different velocities and kinetic energies ---- Collision Theory and Maxwell-Boltzmann distribution ---- Arrhenius equation and Activation energy (b) concentration ---- frequency of collisions and effective collisions
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WHAT IS TORQUE? Torque is a measure of how much a force acting on an object causes that object to rotate. The object rotates about an axis‚ which we will call the pivot point‚ and will label ’O ’. We will call the force ’F ’. The distance from the pivot point to the point where the force acts is called the moment arm‚ and is denoted by ’r ’. Note that this distance‚ ’r ’‚ is also a vector‚ and points from the axis of rotation to the point where the force acts. (Refer to Figure 1 for a pictoral
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Use the knowledge of kinetics to explain each of the following statements. a. An increase in temperature at which a reaction takes place causes an increase in reaction rate . An increase in temperature means increasing the energy of the molecules present. If the molecules has more energy ‚ then more of the them will collide often with enough energy ‚ to overcome the activation energy barrier. causing the reaction to proceed more quickly. b.An addition of the catalyst increases the rate at which
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Experiment 4: Enzyme Kinetics. Results/Discussion Week 1 Part A: Table 1. Enzyme activity for each assay of 4-nitroaniline formation. Rate of 4-nitroaniline formation Name of trial Abs/sec Abs/min M/min mol/min µmol/min #1 0.00003 0.0018 2.05x10-7 2.15 x10-10 2.15 x10-4 # 2 0.00010 0.0060 6.81x10-7 7.15x10-10 7.15x10-4 # 3 0.00020 0.0120 1.36x10-6 1.43x10-9 1.43x10-3 # 4 0.00030 0.0180 2.00x10-6 2.10x10-9 2.10x10-3
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Heat transfer is a discipline of thermal engineering that concerns the generation‚ use‚ conversion‚ and exchange of thermal energy and heat between physical systems. Heat transfer is classified into various mechanisms‚ such as thermal conduction‚ thermal convection‚ thermal radiation‚ and transfer of energy by phase changes. Engineers also consider the transfer of mass of differing chemical species‚ either cold or hot‚ to achieve heat transfer. While these mechanisms have distinct characteristics
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