dilution test, how is it used to determine susceptibility?
I. Define the following:
a. Minimum Inhibitory Concentration (MIC)
b. Zone of Inhibition
J. Whatwere the results of your Kirby-Bauertest for S. epidermidis?...
antimicrobial agent that is capable of preventing growth of the organism undergoing the tube dilution test
b. Zone of Inhibition: this is the area surrounding the high potency disk of a chemotherapeutic agent where growth is inhibited in an agar diffusion test.
J. Whatwere the results of...
Figure 2a-2d show the results of the KirbyBauertest. For both S. lentus and S. epidermidis, zones of inhibition were present for the following antibiotics: Streptomycin, Tetracycline, Erythromycin and Chloramphenicol. Sulfamethoxazole-trimethoprin, Penicillin, and Ampicillin all showed no zone of...
efflux (pumping) of the drug out of the cell before it can damage the micro-organism
Whatwere the results of your Kirby-Bauertest for S. epidermidis? Small zones up to several mm.
Antibiotic Resistant Intermediate Susceptible
Gentamicin against gram-negative bacteria Intermediate...
Title : Identifying Skin Flora
The identification a skin flora was tested in this lab experiment. This was done using the streak plate method , smearing, gram staining and Kirby- Bauer method. The bacteria were observed on agar plates and also under a microscope (gram staining). From...
mechanism of antimicrobial resistance. Penicillin
can be rendered ineffective by an alteration mutation that disrupts the target protein.
The Kirby-Bauertest is an agar diffusion test used to identify bacterial susceptibilities. When S. epidermidis is exposed to antibiotic disc materials of Novobiocin...
bacteria include: Lysol, Basic G, Clorox, and Assist QT. Susceptibility of resistibility of the unknown bacteria to these antibiotics and disinfectants were tested and measured using the chart on page 125 and the Kirby-Bauertest procedures in the Willgoh and Bleakly lab manual as a reference (2...
to increased resistance. The way to determine the effectiveness of a given antibiotic is the subject of this lab. The procedure you will be learning is called the Kirby-Bauer antibiotic sensitivity test, also known as the disc diffusion test. You will be determining the resistance or sensitivity of...
, staphylococcus, tetrad, etc.
Gram stain reaction
Endospore stain results
No spores produced
Identification of Unknown
11. Name of the unknown organism- Genus and species. (10 pts.)
Abstract: The purpose of this lab was to use the Kirby-Bauer method to test the sensitivity of Staphylococcus epidermis of specific areas on a petri dish. The antibiotics were gentamicin, novobiacin, and penicillin. This resembled a culture and sensitivity test that is preformed at...
and the S. epidermidis.
In the test tube with L. acidophilus and MRS broth, a thickened sediment formed and turbidity resulted when the contents in the tube were swirled. Under the microscope I could see large individual microbes.
In the test tube with S. epidermidis, a thick pellicle formed on...
data collection used
Results from the aforementioned testswere determined through observation of the
presence or absence of microbial growth in the agar plate for the Kirby-Bauer method, as
well as in the series of test tubes for MIC. The observation method is the preferred method
Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity
1. Complete the following table on coagulase and DNase activity.
Biochemical ResultsS. aureus S. epidermidis
Coagulase test (+ or –) DNase test (+ or...
disks as well as conditions of incubation, measurement, and interpretation of results. METHODOLOGY
Research Design and Methods
The researchers used Kirby-Bauer Method. Three bacteria: Escherichia coli, Staphylococcus aureus and Salmonella enteritidis were used for the study. Gentamicin was used...
Kirby-BauerTest for Bacterial Resistance to Particular Antibiotics
The Kirby-BauerTest is a standardized method to establish whether a particular strain of a bacterial species is susceptible or resistant to one or more antibiotics. The test is based on the size of a zone of inhibition, or...
. acidophilus will not grow sufficiently in nutrient media.
I. Describe your microscopic observations of the cultures.
Specimens of S. epidermidiswere spherical like in shape and grouped together in a cluster. In the direct stain the specimen was shaded a magenta/purple color.
Specimens of L...
Hudzicki, J. (2012, March 29). Kirby-Bauer Disk Diffusion Susceptibility Test Protocol. Retrieved June 28, 2012, from American Society for...
• Zones of growth inhibition are measured (mm) • Zone size is referenced to CLSI (NCCLS) interpretive chart • Qualitative (categorical) results = S, I, R • Advantages • firmly established method • relatively inexpensive • flexible drug selection
Staphylococcus epidermidis. Specifically, the researcher sought to answer the following sub-problems: 1) What are the active constituents present in Papaya leaves extract? 2) What is the level of effectiveness of Papaya leaves extract on Staphylococcus epidermidis? 3) Is there any significant difference...