Isolation and identification of an unknown bacterium Pillay‚ Esmerelda (209504371) School of Biochemistry‚ Genetics and Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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Constructing Plant Phylogeny Based on Morphological Characteristics and Molecular Data Phylogeny is the study of evolutionary relationships among groups of living and extinct organisms on earth (Campbell et al.‚ 2011). To show evolutionary relationships‚ a phylogenetic tree (a visual representation of the lineages among organisms) is constructed. All phylogenetic trees are hypotheses that are to be tested‚ modified‚ and tested again. One type of tree‚ known as a
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Identification of Unknown Carbohydrates using various biochemical tests Date of experiment: 11/10/2012 Name of Student: Vicki Marshall Introduction A series of biochemical tests was carried out to identify unknown carbohydrates. There were six carbohydrates that needed to be identified; they have been randomly labelled A-F. The carbohydrates are glucose‚ fructose‚ maltose‚ lactose‚ sucrose and starch. There was six tests that were carried out to help identify them‚ these were: Iodine Test
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A Report of Experiment 1 Purification and Identification of An Unknown Mixture Aim To separate a mixture of a neutral (ester‚ alcohol‚ ketone‚ aldehyde or ether) and an acidic compound (phenol or carboxylic acid) or a neutral and a basic compound (amine)‚ and to identify the unknown mixture by physical (M.P. or B.P.) and spectroscopic methods (NMR‚ IR and MS). Method The mixture (sample number: 27) was dissolved in 200 mL of CH2Cl2 and then extracted with 10% NaOH. The acid was removed
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ANALYSIS OF BACTERIA GROWTH Abstract Bacteria can be found everywhere on the earth. Bacteria are single-celled organisms that lack a nucleus‚ mitochondria‚ and chloroplasts and are surrounded by a cell wall containing a peptidoglycan layer. They are defined by their small size and wide range of shapes. In this experiment we separated organisms in a mixed culture to study the colony morphology and physical characteristics and determine which organism was present in higher numbers‚ as determined
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“Fighting Invisible Killers” Scholastic Scope: The Language Arts Magazine January 2014 Edition‚ pages 5-9 Bacteria surround us every day. These little “bugs” are invisible to the eye and most do not harm us. Many are necessary for us to survive‚ like the bacteria in our stomachs and intestines that help us to digest food. But some bacteria are very dangerous to us. Addie Rereich became very sick in May 2011‚ when she was 11 years old. What started as something that her doctors and mother
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All living organism depend on catalytic proteins called enzymes‚ these compound are responsible for metabolism and biochemical regulation of energy‚ there are millions of enzymes and hundreds of enzymes have been identified in the human body as well. All living system possess enzymes as vital components of the body‚ enzymes are found in all organisms. Everyone has a natural pH balance in the body. Enzymes are complex proteins which speed up chemical reactions if this did not happen it would occur
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Physiological Principles for Health and Social Care. Name: Santoya Bennett I.D: P1012646 Group: 50 In this essay I would be writing about the main features of the human body and there functions. The major systems of the human body are Cardiovascular System‚ Digestive System‚ Endocrine System‚ Urinary System‚ Immune System‚ Muscular System‚ Nervous System‚ Reproductive System and Respiratory System. The human body is a complex collection of various systems‚ which work together to make
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Bacteria are the most numerous type of microorganism found in the rhizosphere of the soil. They produce secondary metabolites which are capable of producing antibiotic which eventually inhibit or kill bacteria. The rhizosphere region of the soil is a highly favorable habitat for the proliferation‚ activity and metabolism of numerous microorganisms. The magnitude of this area depends on the plant and the size of the roots that the plant possesses. Bacteria are among the microorganisms living in the
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