In the first part of this lab‚ E.coli cells were transformed with an R-plasmid carrying a tetracycline resistant gene‚ giving rise to tetracycline resistant E.coli strain. This was accomplished through transformation‚ which allowed E.coli to directly uptake the naked DNA molecule carrying the antibiotic resistant gene (1). However‚ in order to take up the DNA and incorporate them into their genome via recombination‚ cells must be competent (1). Therefore‚ E.coli cells which are not competent under
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Worry is to be conscientiously or emotionally blocked by fear . This is how Manuel felt in Gary Soto’s “La Bamba” although he should have been relaxed Gary Soto’s theme for this story is that you should relaxed over things that are just for fun. He expresses this through Manuels emotions‚ Other character’s reactions and Manuels private thoughts. Soto shows our theme by including characters reactions to Manuel’s performance to develop feeling in the characters contributing to
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As kids‚ people make mistakes. It’s inevitable‚ just like any other part of life. In an excerpt of Gary Soto’s autobiographical narrative‚ Soto uses contrasting diction to argue that guilt way eat away at one’s innocence. Soto uses positive diction and vivid imagery to describe himself and the cause of his actions. In the beginning of the passage‚ Soto portrays the image of himself being a good and proper boy by using allusions from the bible and childlike diction. Soto uses phrases like “holy in
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just received their grades on the first test. The instructor has provided information about the first test grades in some previous classes as well as the final average for the same students. Some of these grades have been sampled and are as follows: Student 1 2 3 4 5 6 7 8 9 1st test grade 98 77 88 80 96 61 66 95 69 Final Average 93 78 84 73 84 64 64 95 76 (a) Develop a regression model that could be used to predict the final average in the course based on the first test grade. (b) Predict the final
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Smalley personality profile test: Smalley personality profile test is based on Gary samlleys system‚ It’s an objective test much into the personality of ourselves. This can be done in four segments in which each segment is indicated with an animal such as Lion‚ Bearer‚ Otter‚ and Golden Retriever. Every segment is listed out with 20 personality traits‚ qualities‚ charactaristics‚ e.t.c We gave to go through the lists and select the matchings. Count the selected in each of the four segments and double
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Commentary for “Soldier’s Heart” Author‚ Gary Paulsen The book Soldier’s Heart‚ is based on a true story about a fifteen-year-old boy in Minnesota named‚ Charley Goddard‚ who lies about his age to join the First Volunteers of Minnesota to fight in the Civil War. Some of the events and time sequences are not completely factual‚ but the essential elements of the books story are true. Charley Goddard is a fifteen-year-old boy growing up in the farming community of Winona‚ Minnesota‚ in 1861‚
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One of the most important military techniques used in World War 1 was the use of trench warfare. In the movie‚ All Quiet on the Western Front‚ both the positive and the negative aspects of trench warfare are highlighted. The movie begins with German men hiding behind the trenches as the French Army approaches. As the French march closer‚ the Germans are able to shoot at them‚ but avoid being shot themselves by ducking down in the trenches. This is particularly helpful because it saved lives while
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No one will ever have a perfect life‚ there will always be ups and downs. No matter how hard you try to make your life perfect it will not be‚ but you can make the not so great parts better. Gary Soto’s life was nowhere near perfect‚ from his father’s death to moving and not having money. After his father’s death‚ his life was not great‚ it was shown so much in his regular life and in his professional life. Forgetting about his rough past he was such a brilliant poet and wrote so many short stories
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Caroline Castro‚ AP Language and Composition Gary Soto Essay- A Summer Life February 3‚ 2012 In the well-written autobiographical narrative A Summer Life (1990)‚ Gary Soto delivers an original assembly of aspects from himself as a six-year-old child. Soto asserts the scary realization of wants triumphing over what is ethical and he uses many examples of imagery‚ repetition and a chosen vocabulary to sketch out the ignorance that is evident in a child’s mind. Soto’s purpose is to selectively
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"Oranges" by Gary Soto‚ the narrator recalls a memory from his childhood - the first time he goes on a date with a girl. The boy is young and probably a little scared at first‚ but things turn out well and the date with the girl seems to have an impact on him later in life. We don’t know anything about the narrator when he is older but he seems like a likable person. He tries to make the girl happy in the poem and he seem like someone who doesn’t only think of himself. The first line of the poem
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