involves measuring the absorbance of several concentrations of the pure substance or the "standard" substance determine relationship between concentration and absorbance compared results from unknowns How to use Linear Regression for Generating a Standard Curve? © 2010 by M. Olaveson UTSC 2 BIO A01F-Fall 2010 - ASSIGNMENT # 2 - Preparing a Standard Curve using Excel 2007 Assignment # 2 Analysis of Data from Lab 2-Exercise 2 Table 2.6. Protein in Test Tubes prepared for
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Experiment 2: Absorbance and Spectrophotometry ABSTRACT: This was an investigation into the effects of different wavelengths of light on methylene blue and carmine red on the absorbance value on a spectrophotometer. A spectrophotometer is used to measure light intensity by emitting a single light source through a cuvette of coloured solution. The particles in the solution‚ which are coloured‚ absorb the light depending on how concentrated it is and this produces an electronic reading from the photometer
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report investigates the absorbance of methylene blue and carmine red using a spectrophotometer to determine the absorption spectra of both solutions. The concentration of the unknown solution of methylene blue was found to be 1.07 x 10-5 M by using the molar extinction coefficient‚ with absorption of 0.547. It was also found that the results concluded confirmed beer’s law with an R2 value of 0.9989. Introduction Spectrophotometry is the quantitative measurement of the absorbance or transmittance properties
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by Atomic Absorbance Spectroscopy Abstract: The purpose of this experiment is to analyze a sample containing copper and zinc in trace amounts‚ where standard addition method was used and the instrument used is the atomic absorbance spectroscopy. The amount of copper obtained was 0.569 ppm ± 0.015 and for zinc we obtained 0.42 ppm ± 0.0027. Introduction: This method implies the excitation of particles in the solution sample and determination of the amount of radiation liberated from that particle
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According to the results‚ there was no significant difference between the filtered 100% water composition‚ and the filtered 90% water 10% soap composition. However‚ based on fFigure 2‚ the Average of Absorbance graph‚ showed that growth of the algae for the 100% water composition was observed to be slightly higher than the 90% water 10% soap composition. Meaning the soap may have slightly affected the growth of the algae‚ in some way. One possible‚ reason for a slightly higher growth for the filtered
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Concentration The Problem In many colleges over 8% of the students report problems concentrating on their studies. Most of these students blame outside distractions for their problems. Many research studies manipulating noise levels and distractions have found that such disturbances may increase‚ decrease‚ or not even affect concentration. These researchers have therefore concluded that distracters don’t cause concentration problems directly. It is the way the distracters are interpreted
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you to compare quantitavely the glucose concentration in fresh samples of orange‚ lemon and grapefruit juice. Aim: The aim of this investigation is to produce a set of data which will enable comparison of the glucose concentrations of orange‚ lemon and grapefruit juice. Research: Benedict’s Reagent is a mixture of sodium compounds and copper sulphate. The copper ions are reduced by fructose (a reducing sugar) to form copper oxide‚ thus turning it from blue to a brick red/orange precipitate
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Out of clutter‚ find Simplicity. From discord‚ find Harmony. In the middle of difficulty lies Opportunity. ~Albert Einstein A determined person doesn’t find it hard to succeed; they find it hard to stop trying." --- Allen Steble The entrepreneur is essentially a visualizer and actualizer… He can visualize something‚ and when he visualizes it he sees exactly how to make it happen. ~Robert L. Schwartz "Friendship is born at that moment when one person says to another: "What!"
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Mass Determination of Ca‚ Fe and Zn in a multivitamin using Atomic Absorbance Spectroscopy (AAS) and X-Ray Fluorescence Spectroscopy (XRF) ABSTRACT: The purpose of this project was to determine the amount of calcium‚ iron and zinc present in an over the counter multivitamin. This mass was determined using atomic absorption spectroscopy (AAS) and X-ray fluorescence spectroscopy (XRF). For both analytical techniques‚ the method of standard additions was used to compensate for matrix effects.
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Experiment 2: Estimation of protein concentration Introduction Protein assays are designed to measure the total protein in a solution. Protein assays are quantitative if the protein to be assayed is available in sufficient quantity such that one is able to use it to create a standard curve. If this cannot be achieved‚ then a standard protein‚ such as albumin‚ may be used for a standard curve with the understanding that the results on the unknown protein are semi-quantitative. Since most proteins
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