Cellular Respiration and Fermentation: Experimenting With CO2 and Redox Reactions Julius Engel; Section 8 Abstract In this experiment‚ the subjects of study were fermentation‚ mitochondrial respiration‚ and redox reactions. In the first experiment‚ yeast was grown in various carbohydrate solutions at various temperatures. In the second experiment‚ succinate was added to various samples of a mitchondrial suspension‚ DPIP‚ and a buffer. Then after two blanks were used‚ the samples
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What amount of amylase is the best to produce a better reaction with starch? A 1% solution of amylase‚ made from 0.15g of amylase added to 15ml of water‚ a 2% solution of amylase made of 0.3g of amylase added to 15ml of water‚ and a 0.5% solution of amylase made of 0.75g of amylase to 15ml of water were created. A 0.25% starch solution was also created‚ and 3ml was added to each of the 4 test tubes. 1 drop of iodine was added to each test tube. 4ml of the 1% solution was added to the first test
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Jolyne Piet CHM-221L-02 Lab #2: Experimental Design Isolation of Sucrose: 3.01 g Panacetin were weighed in a 125-mL Erlenmeyer flask‚ and 51mL dichloromethane were added to partially dissolve the Panacetin. The insoluble portion was gravity filtered and air dried to yield 0.45 g of sucrose (15.0 % of original Panacetin). Isolation of Aspirin: The organic filtrate was extracted through a separatory funnel with 32 mL 5% sodium bicarbonate to produce an aqueous layer and a dichloromethane layer
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Design: Investigation of Fermentation Introduction Introduction Yeast (Saccharomyces)is a single-celled microorganism in the Fungi family. It anaerobically respires sugars to produce ATP‚ as well as the waste products ethanol and carbon dioxide gas. This process is known as fermentation. There are various factors that affect the rate at which yeast respires. Aim To investigate the effect of concentration of table salt (sodium chloride) on the rate of fermentation of sucrose using yeast‚ measured
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strove to determine how effective different sugars were in fermentation by measuring their CO2 emissions. An increased CO2 production implies the substrate is undergoing glycolysis more often‚ resulting in increased ATP synthesis. For a more accurate interpretation of the results‚ glucose acted as a positive control‚ displaying the most efficiency in fermentation as shown in Figure 1 and Figure 2‚ while ethanol‚ which is a byproduct of fermentation‚ acted as a negative control‚ producing no CO2. These
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Fermentation of lignocellulosic hydrolysates involves the conversion of sugars to ethanol which is mainly performed by bacteria or yeast. The organism chosen should possess certain characters in terms of tolerance I‚e towards inhibitors ‚sugars and ethanol concentrations in the hydrolysates and should also withstand higher temperatures and lower pH and with minimal by product formation [161]. Fermentation is the key component where advancement in technology plays key role and is required to be feasible
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It about modified starch Journal of Agricultural Science Vol. 2‚ No. 2‚ June 2010 requirements are properties such as viscosity‚ resistant to shear‚ low pH and high temperature. Accordingly‚ varieties of modified starches are used in food industry. Table 1 lists some of the modified starches that can be prepared from different sources to meet the marketing-related requirements. Today‚ modified food starch is a food additive and limits of its modification‚ use and labeling are clearly defined
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Treating Starch How are starch and cellulose treated to allow them to be used in yeast? Starches: · All potable alcohol and most fermentation industrial alcohol is currently made principally from grains. · Fermentation of starch from grain is somewhat more complex than fermentation of sugars because starch must first be converted to sugar and then to ethanol. · Starch is converted enzymatically to glucose either by diastase presents in sprouting grain or by fungal amylase. · The resulting
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Fermentation Fermentation is a natural process that has been going on in nature since before humans existed. For centuries we have been practicing food fermentation‚ knowingly or unknowingly. Every food culture in the world throughout history has been using fermentation in their food in some way. Bread making originated in Egypt 3500 years ago. Fermented drinks were being produced and consumed in Babylon(now Iraq) 7000 years ago. China is thought to be the birth place of fermented vegetables. A
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or a dark purplish black‚ then the test for starches is positive. If DI water‚ and sucrose are tested for proteins using biuret reagent‚ then they will show a negative result. To explain‚ both DI water‚ and sucrose alone do not contain a trace of protein. In addition‚ deionized water is commonly used in experiments as a negative control. In both experiments‚ if the DI water is tested for a protein or starch‚ then it will come back as a negative. If milk solution‚ and 50% egg white solution are
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