and subjected to qualitative tests for identification. It is suggested that culture #72 is an example of Serratia marcescens and Micrococcus luteus. There were ten bacteria species possibilities: Stahylococcus epidermidis‚ Bacillus subtilis‚ Escherichia coli‚ Serratia marcescens‚ Sarcina lutea‚ Pseudomonas fragi‚ Micrococcus luteus‚ Alcaligenes faecalis‚ Clostridium sporogenes‚ and Micrococcus roseus. There were several qualitative tests that could be conducted to determine the identity of the unknown
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References: 1. R. S. Horvath and M. E. Ropp. 1974 “Mechanism of Action of Eosin-Methylene Blue Agar in the Differentiation of Escherichia coli and Enterobacter aerogenes”.International journal of systemic bacteriology. April 1974‚ p. 221-224 2. Dagny Jayne Leininger‚ Jerry Russel Roberson‚ Franc¸ois Elvinger. 2001 “Use of eosin methylene blue agar to differentiate Escherichia coli from othergram-negative mastitis pathogens”. J Vet Diagn Invest 13:273–275 3. Hiroshi Fujikawa‚ 1994. “Diversity of the growth
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maize‚ bacteria Erwinia uredovora and Escherichia coli. The insertion of phytoene synthase (psy) gene from daffodil or maize and phytoene desaturase (crt I) from Erwinia uredovora into rice genome reintroduced carotenoid biosynthetic pathway in the endosperm that was absent before. This gives the ability of making β-carotene (precursor of Vitamin A) in the endosperm instead of vegetative tissue of rice plants. The phosphomannose isomerase (Pmi) gene from E. coli helps in positive selection of intended
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may enter the wound from the air in the OR(operating room)‚ or from the instruments or surgeon(s) that come into contact with the wound. Skin bacteria are1 always present despite the throughness of the preparation of the skin. The largest inoculums of bacteria at the surgical site occur when the operation involves a body structure that ordinarily is heavily colonized by bacteria‚ such as the bowel. Procedures involving the female genital tract will encounter 106 – 107 bacterial/ml. 29
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type of genetic transformation that was used in lab and mainly used due to the single celled nature of bacteria. In this lab‚ the engineered pGLO plasmid is integrated into E. Coli bacteria‚ and adds the genes which code for the proteins GFP in the modified bacteria’s genome (Hanahan‚ Studies on transformation of Escherichia coli with plasmids‚ 1983). To see the reaction of this plasmid on the cells‚ bacteria treated with the plasmid were grown on two separate agar plates containing LB nutrient broth
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Gene Expression Introduction: Escherichia coli are capable of using lactose as their sole carbon source. E. coli produces the enzyme β-galactosidase to digest the lactose into glucose and galactose. However‚ it would be inefficient to produce enzymes when there is no lactose available‚ or if there is a more readily-available energy source available such as glucose. Therefore there must be something controlling the expression of this enzyme. The purpose of the experiment is to determine whether
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optimum temperature for growth of Escherichia coli 0157:H7 in Trypticase Soy Broth. This grew poorly in the temperature range of 44 to 45.5 °C which is generally used for recovery of E. coli from foods. Bettelheim (1998) designed Rainbow Agar O157 for the isolation and identification of EHEC. In this medium E. coli O157 was characterised by black colonies whereas O113 and some other EHEC strains were mauve‚ red or pink and indistinguishable from other strains of E. coli. Manafi and Kremsmaier (2001)
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Lahela Correa 12/08/2009 Microbiology 140 Matthew Tuthill Unknown Lab Report Introduction There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient‚ so as to know how it can be treated‚ to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification
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the streak plate procedure‚ used to isolate pure colonies of bacteria‚ and viable plate count methods. The latter involves serial dilution and spread plating of bacteria on agar plates. Materials required per pair • One 10 ml liquid culture of Escherichia coli BL21 (see prior preparation) • A sample of Yakult (approximately 5 ml) • Marker pens to label plates & bottles • Sterile plastic loops for streaking bacteria (up to 20 per pair) • Sterile plastic spreaders for spreading bacteria (4 per
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au/phb/hprot/food/fhpp/hfp.html> Electric Library. Ann Hollingsworth. "Food Safety." 2000. 18 Jan. 2001. <http://www.elibrary.com/s/edumark/g...bigchalk.com:US;EL&dtype=0~0&dinst=> Electric Library. "E. Coli Now in Our Alfalfa Sprouts?" Vol. 21‚ Medical Update‚ 1998. 17 Jan. 2001 Kill E. coli 0157:H7 and Salmonella" 1999. 18 Jan. 2001. <http://www.ars.usda.gov/is/pr/1999/990601.htm> Electric Library. "HHS Initiaties to Reduce Foodborne Illness." 1999. 18 Jan. 2001. <http://www.elibrary.com/s/edumark/
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