Matthew Saldanha Bio DCP lab-Catalase experiment Aim: To investigate enzyme kinetics‚ using different concentration of the enzyme. Hypothesis: The assay system used in the lab consists of a filter paper disc coated with the enzyme and the dropped into a papercup of substrate (Hydrogen Peroxide). As the hydrogen breaks down the hydrogen peroxide into hydrogen and oxygen gas‚ the bubbles of oxygen gas collect underneath the filter and make it
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Polymerase chain reaction The Polymerase chain reaction (PCR) is a biochemical technology in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude‚ generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis‚ PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. These include DNA cloning for sequencing‚ DNA-based phylogeny
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There are COX enzyme isoforms include COX-1 and COX-2. (Figure 3.8) These enzymes originate from different genes and have distinct tissue-specific expressions and biologic functions. COX-1 is constitutively expressed in most tissues and believed to responsible for the production of prostanoids in homeostasis‚ such as gastric epithelial cytoprotection and renal blood flow maintenance. COX-2 expression is inducibly expressed only at marginal levels under condition in many tissues and is upregulated
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Rates of Reaction Coursework Aim: To find out how different concentrations of sodium thiosulphate (Na S2 O3) affects the speed of its reaction with Hydrochloric acid (HCL). Introduction When Sodium Thiosulphate and Hydrochloric acid react they produce a cloudy precipitate. Both of the chemicals are clear solutions and they react together to form a yellow precipitate of sulphur‚ the equation for this reaction is: Na2 S2 O3 + HCL‚ H2 O + NaCL + SO2 + S Sodium Thiosulphate + Hydrochloric
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Jolly Nguyen 11C Conditions needed for effective enzyme action Aim: To investigate the activity of enzymes and how might the activity be effected in different conditions. Hypothesis: Temperature rises and change of substrate concentration may cause denaturation of the protein of enzymes. So as the temperature rises the amount of active enzyme progressively decreases‚ and the rate is slowed. Exposure to heat causes atoms to vibrate violently and this disrupts bonds within globular proteins
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Experiment 3: Wittig Reaction Introduction This experiment performs a modified Wittig reaction using a phosphorus-containing Hornes-Emmons-Wittig reagent to generate an enolate anion of trimethyl phosphonoacetate instead of a phosphorus ylide. The methyl trans-4-methoxy cinnamate produced is then analyzed using melting point and 1H NMR spectroscopy. Theory The Wittig reaction prepares alkenes from carbonyl compounds by attacking a phosphorus ylide with a nucleophilic carbon atom stabilized by
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Aldol In this preparative lab‚ an aldol (trans-p-anisalacetophenone) was produced from the reaction between p-anisaldehyde and acetophenone with the presence sodium hydroxide. The reaction also showed the importance of an enolate and the role it played in the mechanism. Sodium hydroxide acts as a catalyst in this experiment and is chosen because of its basic conditions and pH. The acetophenone carries an alpha hydrogen that has a pKa between 18 and 20. This alpha hydrogen is acidic because of its
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| |Four |Experiment One: Control | |Five |Experiment Two: Temperature Change | |Six |Experiment Three: Alteration of PH | |Seven |Experiment
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Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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Cells are the structural and functional units of all living organisms; each specialised to perform dedicated duties throughout their life span. All living organisms can be sorted into one of two groups depending on the fundamental structure of their cells. These two groups are the prokaryotes and the eukaryotes. A Prokaryote cell is the first form of cells for many millions of years until the eukaryote cell evolved from the prokaryote cell‚ which created life. Prokaryote cells are organisms made
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