EXTRACTION OF INVERTASE FROM YEAST AND EFFECT OF pH ON ENZYMATIC ACTIVITY Jenelle C. Faustino‚ John Gambit B. Garcia‚ Fatima S. Jusay‚ Oliver Alexander B. Lao and Eunice L. Licudine Group 4 2 E Medical Technology Biochemistry Laboratory ABSTRACT Enzymes are substances that are produced by living organisms and act as catalysts in order to speed up or chance a chemical reaction without changing itself at the end of the reaction. Invertase was extracted first from baker’s yeast. Determination
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Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab‚ click on the Experiment button to begin the first assignment. This assignment is designed to help you become
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Enzyme Lab Using Jello INTRODUCTION: Enzymes are known as protein catalysts. The name protein catalyst suggests that most enzymes are made of proteins. A catalyst is a substance that speeds up chemical reactions without being consumed in the process. (Giuseppe‚ M 2002‚ p.69). After a reaction has been catalyzed‚ the catalyst can be used again to catalyze the same reaction. Enzymes reduce the activation energy (minimal energy) it takes for a reaction to take place. Enzymes can either catabolize
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A KINETIC STUDY OF AN IODINE CLOCK REACTION PURPOSE To investigate the kinetics of the reaction that occurs between iodide and persulfate ion. You will: (1) determine the rate law‚ (2) determine the numerical value of the rate constant at room temperature‚ (3) explore the effect of temperature on the reaction and determine the activation energy (Ea)‚ and (4) investigate catalytic activity of selected metal ions on the reaction. INTRODUCTION Reaction times vary from picoseconds (10-12 seconds)
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Aim: The aim of the experiment is to test the effect temperature has on the activity of the enzyme rennin. Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC‚ which is the body temperature‚ where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working. Introduction:Enzymes are
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2013 March 17‚ 2013 Name : Ryan annasdass arokiasamy ID : 1206875 Group Members : Chan Pei Qie‚Chong Ven Yen Name : Ryan annasdass arokiasamy ID : 1206875 Group Members : Chan Pei Qie‚Chong Ven Yen experiment 19 kinetics : the study of a chemical reaction experiment 19 kinetics : the study of a chemical reaction Results Part A [I-] / mol dm-3 | [S2O82-] / mol dm-3 | [S2O32-] / mol dm-3 | Time /s | Rate of I2 formation / mol dm-3 s-1 | 0.2 | 0.2 | 0.01 | 1.25 | 0.1600 | 0.2
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Caselet 1 Kinetic Engineering Limited (KEL) has created a buzz in biking circles with its announcement of introducing the 250 cc cruiser Aquila of Hyosung Motors of Korea. It has generated curiosity and interest among bike enthusiasts in the country. The bike is being promoted as a ’real’ cruiser. It is fitted with a 250 cc. v-twin‚ oil-cooled engine with four valves per cylinder. It has an output of 26 bhp. with top speed of over 130 kmph. The company is the first to introduce this high-end bike
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milk Introduction Enzymes are globular protein‚ responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon‚ hydrogen‚ oxygen and nitrogen (Gunsch‚ 2012). The role of enzyme is to act as catalysts‚ substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done by lowering the activation energy required to start a reaction. Enzymes are specific in
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----------------------------------------------------- Abstract The interactions of Mg2+ and Zn2+ ions in the activation of non-specific tissue alkaline phosphatase were investigated using crude extracts of rat kidney. Activation of alkaline phosphatase by the metal ions was accompanied by changes in the kinetic parameters of pnitrophenylphosphate hydrolysis. The results suggest some synergistic interactions between Mg2+ and Zn2+ ions in promoting the hydrolysis of p-nitrophenylphosphate by alkaline
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iodine solution is added and flushed. The higher the amylase activity‚ the more the starch will be broken down. Hence‚ a larger and clearer zone will be observed. Thus the diameter of the clear zone can show the activity of the amylase. In this experiment‚ the independent variable is the types of washing powder in the solution. The dependent variable is the diameter of clear zone on agar plate. The controlled variables are the concentration and volume of washing powder solution added into well‚ size
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