62 Iodine test for starch Amount of starch remaining Enzyme activity level Dark blue-black All None (0) Blue Most Low (1) Light brown Some Moderate (2) Gold None High (3) Part 1: Effect of Enzyme Concentration 1. Label five test tubes 1-5. Place 4 mL of 1 % starch in each of the first four test tubes. Place 4 mL of amylase solution in the fifth tube. Place all of the tubes in the 37°C water bath for 5 minutes. Obtain 5 clean droppers and label them 1-5. (To avoid contamination of these solutions
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Lab 6: Enzymes . header Purpose: The purpose of this lab is to test for enzyme activity‚ look at enzyme specificity‚ and how temperature affects enzyme activity. Time need to perform this lab: approximately 3 hours Preparation FIRST: Read the lab in its entirety TWICE before you begin. You will perform the experiment‚ write your lab report and include the answers to the additional 4 questions within the text for full credit on this experiment. Materials: •3% hydrogen peroxide •a
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Environmental Parameters of Enzyme Activity Alex Rocha Texas State University Abstract If you’ve ever left a cut up apple out for long‚ you’ll notice that after a while‚ it will turn brown. The reason for this is an enzyme named catechol oxidase‚ a ubiquitous plant enzymes containing a dinuclear copper center (Klabunde‚ Eicken‚ Sacchettini‚ & Krebs‚ 1998). In this experiment‚ we used two different chelators‚ ethylene diamine tetraacetic acid and phenylthiourea to test which would stop
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Catalase Enzyme Lab Samiya Hussein March 9‚ 2012 Introduction In order to receive the necessary amounts of energy required for daily function‚ the digestive system must break down proteins‚ fats and carbohydrates. In doing so‚ the body produces poisonous chemicals; however‚ the cells aren’t harmed. This is because enzymes are used to break down these chemicals. The name of the enzyme that was the main focus of the lab is catalase. Catalase is responsible for catalyzing hydrogen peroxide
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Enzymes are biological catalysts that operate at the temperatures‚ pressures‚ and moderate pH values found in living organisms. They help in a faster chemical reaction in our human body. Besides this‚ enzymes also play a huge role in various industries. The following paragraphs will talk about how various enzymes are used in various industries : The Dairy Industry and Medical Industry. In the dairy industry‚ the enzyme Protease is used widely. While making cheese‚ protease helps coagulate milk
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____________________ Immobilizing an enzyme provides various analytical benefits‚ and can be done in a myriad of ways‚ with the most common being entrapment. For this study peroxidase (from horseradish)‚ an enzyme that catalyzes the cleavage of hydrogen peroxide into water‚ was entrapped within a polyacrylamide gel matrix. The gel matrix was formed by the addition of methylene bis-acrylamide (a cross linking agent) to acrylamide. The immobilized enzyme was then tested via spectrophotometric assay
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Enzymes- reduce the amount of energy required for the reactions they catalyse [accelerate]. -thus‚ increasing the rate of reactions that occur in living organisms. WITHOUT enzymes : metabolism would be so slow at body temp. that insufficient energy would be available to sustain life. -Many enzymes are “Intracellular”- used within the cell that produced them e.g. enzymes in respiration & photosynthesis -Others are “extracellular”-they act outside the
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Cellular Biology furthers technology-Enzymes in the Dairy Industry Since ancient times‚ enzymes have played an important role in food production. Especially in the diary industry‚ some enzymes are required for the production of cheese‚ yogurt‚ and other dairy products‚ while others are used in a more specialized fashion such as improving texture or flavour of the product. Enzymes are used to catalyze the desirable reactions in industrial processes. Today‚ enzyme applications in such processing get
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Dehydrogenase catalyzed reaction. 6. To determine the effect of heat on a Xanthine Dehydrogenase catalyzed reaction. Introduction: Enzymes are specialized biologically made proteins that have catalytic functions that are essential for the maintenance and activity of life (Amano 2007). All living cells have enzymes but they are different‚ they depend on the concentration of enzymes that are found in the organelles. A catalyst is a substance that speeds up a chemical reaction but remains unchanged itself
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Dana Calderone Responses of Enzyme Activity from pH and Concentration Abstract Enzymes are the key to many of the chemical reactions that our bodies depend on to live. Without enzymes‚ we would not exist. These biological catalysts speed up the reactions as well as reduce the amount of activation energy needed to complete the process. Knowing how important enzymes are to us‚ it is important to realize what they require to function. They need select conditions and rates to work right. These conditions
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