being digested with EcoRI restriction endonucleasse. Procedures: λ DNA and puC18 DNA were put into two tubes respectively. Then‚ EcoRI buffer‚ EcoRI enzyme and deionized water would be put into both tubes. EcoRI enzyme was the restriction enzyme that cut the DNA at the specific sequence. The EcoRI buffer enhanced the stability of many enzymes and binds contaminants that may be present in DNA preparations. DI water was used to bring the solution into a required volume for gel electrophoresis.
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1. Why are spindle fibers important for mitosis? (5 points) Spindle fibers work as a path for chromosomes to travel(Campbell‚ 2009). 2. State the four bases that make up DNA. (4 points) a. (A)-Adenine b. (G)-Guanine c. (T)-Thymine d. (C)-Cytosine (Campbell‚ 2009). 3. What are the two base pairs? (2 points) a. AG b. CT 4. Answer the following questions: a. Define the term crossing over. (3 points) The phase in meiosis where chromosomes exchange segments of genetic materials (Campbell‚ 2009). b. Explain
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to gel and this experiment is aimed to figure out why. Bromelain is an enzyme the breaks the chains in proteins making them inactive and pineapple are known for having it. In this experiment we tested other factors that pineapple contain to pin point what the culprit was. It was discovered the Bromelain was actually the reason that fresh pineapple won’t gel but if you boil the pineapple or pasteurize it‚ it denatures the enzyme‚ allowing it to gel. Introduction: Why does canned pineapple
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BioLab3 Lab Report 5 Enzymes Student Name: Cooper Lyon I. Enzyme Structure and Function EXERCISE 1 – Preparation of an enzyme activity standard At five minute intervals over the next fifteen minute period‚ record the color intensity of the solution of each test tube. Time (min) Tube S1 Potato Extract + Catechol Tube S2 Potato Extract + Water Tube S3 Catechol + Water 0 Shade of Yellow Clear/Milky Clear/Milky 5 Shade of Yellow Clear/Milky Clear/Milky 10 Orange Clear/Milky Clear/Milky 15
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Question Give an account of how enzymes work with particular reference to factors that affect their activities. Answer All living things require catalysts to catalyse the reaction which they carry out to survive. Enzymes are the catalysts of biological systems without enzymes reactions in cells would occur all much to slow a rate and the organism would not survive. Most enzymes‚ with the exception of some RNA catalysts‚ are proteins. They often require a co factor such as zinc or iron‚ or a complete organic
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An enzyme is a tertiary globular protein. The function of an enzyme is to lower the activation energy of either the creation or breaking apart of a chemical bond. By lowering the activation energy of this process‚ the reaction of bonding‚ or in this case breaking apart‚ is sped up. An enzyme breaks apart the substrate in the active site of the enzyme; this is where the magic happens. Substrate is what is being broken apart by the enzyme. In this case‚ the enzyme is catalase and the substrate is hydrogen
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PSBPP103 Differential Gram’s staining EXPERIMENT NO. 1 AIM: THEORY: Page No: C1 Date: To Gram stain the given bacterial suspension and to differentiate between gram positive and gram negative organism. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. To study their properties and divide microorganisms into specific groups for diagnostic purposes
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The Activity Of The Enzyme Catalase A Level Biology Project Aims This is an experiment to examine how the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase. Background Information Enzymes such as Catalase are protein molecules which are found in living cells. They are used to speed up specific reactions in the cells. They are all very specific as each enzyme just performs one particular reaction. Catalase is an enzyme found in food such as
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How Enzymes work in the home and in industry essay This essay aims to explore the ways on how enzymes are used in home and in industry‚ and it aims to explain the advantages and disadvantages of using enzymes in the home and industry. An enzyme is a protein that is formed by the body that acts as a catalyst to cause a certain desired reaction. Enzymes are very specific. Each enzyme is designed to initiate a specific response with a specific result. Firstly‚ the AQA Science Biology textbook published
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experiment is to test the effect temperature has on the activity of the enzyme rennin. Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC‚ which is the body temperature‚ where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working. Introduction:Enzymes are made up of proteins which
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