PROCEDURE: Part A (Effect of temperature on growth) 1) 15 tubes of glucose broth are provided and one set of 3 tubes are inoculated with each of the following cultures; Escherichia coli‚ Pseudomonas fluorescens‚ Micrococcus luteus and Saccharomyces cerevisiae. The last served as control. 2) One of the three tube of each culture is incubated at each of the following temperature: * 4°C * 37°C * 55°C 3) All the tubes are incubated within 5 minutes after inoculating. The turbidity
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The Effect of Temperature on the Rate of Diffusion Damsel C. Bangcal Aira May V. dela Cruz Jacqueline L. Lacuesta Richelle Jem P. Jobog Group 1 Section U-2L November 17‚ 2014 ___________ 1A research proposal submitted in partial fulfillment of the requirements in General Biology I Laboratory under Proj. Joan O. Adajar‚ 1st semester‚ 2014-2015. INTRODUCTION Molecules undergo constant motion and move in regions with a higher concentration to a lower concentration
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Topic 2 – Cell membranes Reading Chapter 5 Objectives Functions of cell membranes The fluid mosaic model The lipid bilayer – phospholipids‚ glycolipids and sterols Properties of the lipid bilayer Membrane proteins – the ‘mosaic’ in the model Solute movements across membranes BIO 1140 – SLIDE 1 http://www.nikonsmallworld.com/gallery/year/2006/id/751 Functions of cell membranes – an overview Fluid mosaic model Singer & Nicolson 1972 Fluid lipid molecules in which
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Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL‚ 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start
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Effect of Catalase at Different Temperatures Abstract The role of this experiment is to determine the effect of temperature on enzymatic activity. The results of the experiment were the colder the temperature the slower the reaction rate and the hotter the temperature the faster the reaction rate. Introduction Enzymes are chemical substances found in living cells and they act as catalysts of the various chemical reactions that occur in them.(Preszler‚ 2012) They bind to substrates that have
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phosphorylase in particular) are affected by varying its temperature before introducing it to the substrate it will be reacting with. A catalyst (enzyme) is a substance that changes the rate of a reaction; for a reaction to take place at all‚ the enzyme must first come into contact with the substrate. Enzymes are subject to a number of factors which effect how fast they can cause a reaction with a substrate; these factors include temperature‚ pH levels‚ chemical substances and the concentration of
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Investigating the Effect of Temperature on Enzyme Activity Almost all chemical reactions that occur in living organisms are catalyzed by enzymes. Many factors in a cell’s environment affect the action of an enzyme. In this investigation‚ you will design an experiment to determine the effect of temperature on an enzyme-catalyzed reaction. You will complete an entire lab write-up using the CHS LAB REPORT FORMAT. Fundamental Question How does temperature affect the rate of an enzyme-catalyzed
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test for the effects of temperature on catalase activity. Introduction: Enzymes are proteins that act as catalysts and help complex reactions to occur everywhere in life. Enzymes affect a chemical reaction by acting as a catalyst‚ which is to say‚ it speeds up the reaction. However‚ extreme ph and temperature hinders enzyme function. Optimal activity of an enzyme is the optimal environment that an enzyme requires to function‚ which means having the optimum ph and temperature to catalyze a
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Aim How temperature affects membranes: investigating the effect temperature has on Beta vulgaris membranes when submerged in a range of different temperatures for one minute. Background Information A cell membrane is made up of a phospholipid bilayer formed by phospholipids that have a hydrophilic head and a hydrophobic tail. The phospholipids arrange themselves in order that the heads are exposed to water and the tails are not thus forming the bilayer. When the temperature increases molecules
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