Testing Catalase Activity Prepared by Anel Behric For professor Goodrow General Biology Lab Mohawk Valley Community College 10/14/2014 6:00 PM Introduction: This experiment was conducted to examine the breakdown of substrate hydrogen peroxide (H2O2) by catalase‚ which is a specific enzyme that breaks down substrates of (H2O2). Molecules are in our bodies and nature. They move around constantly which causes them to be part of random collisions‚
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Lab 1: Effect of pH on sodium benzoate‚ a food preservative Sheikh M Zakaria Person no. 36295651 TA: Synthia Gratia Date of Submission: 05/29/13 Abstract Sodium Benzoate is a common food preservative used in food products such as jams and jellies‚ soft drinks‚ pickles‚ condiments etc and in tinned products in the market. This experiment aimed to determine whether benzoic acid is formed from it’s superior soluble form sodium benzoate in stomach acid‚ which is simulated by HCl (pH=2). It has
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Aim: To investigate the effect of hydrogen peroxide concentration on enzyme catalase Indroduction: Enzymes are proteins. They function as biological catalysts. They lower the energy barrier of a reaction so that the reaction can take place at body temperature. Also‚ they can speed up Metabolic reactions without being changed or used up. During a reaction‚ an enzyme molecule combines temporarily with the substrate. When the reaction is complete‚ the enzyme molecules returns to its original dorm
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4U1) Nicole Mikulis Unit: Biochemistry Sept 14 2012 Lab: Effect of temperature and pH on catalase activity BACKGROUND Catalase is an enzyme that detoxifies chemicals that might harm the cell such as hydrogen peroxide (H2O2). The enzyme breaks H2O2 into water and oxygen. The production of the oxygen gas bubbles serves as evidence that the catalase enzyme is working. As catalase is breaking the bonds between H2O2‚ it is releasing energy in the form of heat which
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Investigating pH Aim: To demonstrate dissolved carbon dioxide changing the ph level of substances. Materials: * 2 beakers * 2 straws * Stop watch * Distilled water * Lime water * Litmus Paper Method: 1. Pour 100ml of Distilled water into the first beaker‚ and label said beaker. 2. Pour 100ml of Lime water into the second‚ also Label beaker. 3. Test the two liquids with litmus paper and note the ph level‚ before any carbon dioxide has been introduced. 4. Place a straw
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[Name] [Teacher] [Course] [Date] Catalase Lab Report An enzyme is something that helps to speed up a chemical reaction. The enzyme changes from reaction to reaction‚ but it always has the same impact. However‚ certain variables may cause the enzyme to have a more or less significant impact on the speed of each reaction. One of these variables that changes the effectiveness of an enzyme is temperature. There is an optimal functioning temperature for each enzyme in each reaction‚ depending on
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Investigation into how pH levels affect Catalase activity in potato samples – (enzymes) Introduction: The aim of this investigation is to understand and monitor the effects pH levels have on catalase activity within controlled potato samples. Catalase is a type of enzyme that is found widespread among organisms that grow in the presence of oxygen (almost all living things) its specific use is to speed up the decomposition of Hydrogen peroxide (H2O2) within cells. Hydrogen peroxide is a bi-product
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An investigation to see how much oxygen is given off when different concentrations of catalase are added with hydrogen peroxide. Aim: To see if changing the concentration of catalase (found in celery) with hydrogen peroxide affects the amount of oxygen given of. Background Information: (Hydrogen peroxide - H2O2 1/2O2+H2O) Enzymes: Hundreds of chemical reactions happen simultaneously inside living cells and it’s the job of enzymes to control and regulate the various metabolic
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Abstract The aim of this study was to test the rate of reactivity of the enzyme catalase on hydrogen peroxide while subject to different concentrations of an inhibitor. The hypothesis was that hydrogen peroxide will be broken down by catalase into hydrogen and oxygen‚ where a higher concentration of inhibitor will yield less oxygen‚ resultant of a lower rate of reaction. Crushed potato samples of equal weight were placed in hydrogen peroxide solutions of various temperatures. The results showed
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known as catalase facilitates this decomposition reaction. The catalase enzyme acts as catalysis‚ helping lower the energy needed to activate the reaction while the enzyme itself is not affected. Catalase is a digestive enzyme used to break down hydrogen peroxide‚ which is a normal byproduct of cellular respiration. The reaction could take place without the help of catalase‚ but it would happen a lot more slowly because more energy is needed for the reaction. This lab demonstrates the effect the enzyme
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